Geminiviridae

Genus: Becurtovirus


Genus: Becurtovirus

Distinguishing features

Although the biological properties of known becurtoviruses are similar to those of curtoviruses, they share only ~60% genome-wide sequence identity. The unique feature of the becurtovirus genome is that the CP is curtovirus-like, whereas the Rep is most closely related to those of mastreviruses. Additionally, becurtoviruses (and eragroviruses) have a unique nonanucleotide at the v-ori that differs from those of all other geminiviruses (Yazdi et al., 2008).

Virion

See discussion under family description.

Genome organization and replication

The becurtovirus genome comprises three overlapping ORFs (V1, V2, and V3) in the virion-sense strand and two complementary-sense ORFs (C1 and C2) (Figure 1.Becurtovirus) (Varsani et al., 2014). The V1, V2 and V3 ORFs are analogous to their curtovirus counterparts, encoding, respectively, the coat protein, movement protein and a protein involved in regulating the ss/ds DNA ratio. The C1 and C2 ORFs, however, are more related to the mastrevirus ORFs occupying the same position in the genome. Furthermore, in contrast to curtoviruses, becurtoviruses lack C3 and C4 ORFs (Yazdi et al., 2008). Based on the evidence from mastreviruses, it is likely that the Rep ORF is expressed from spliced transcripts, and contains an intron sequence. As is the case with the eragroviruses, becurtoviruses have a "TAAGATTCC" nonanucleotide sequence at the v-ori.

Figure 1.Becurtovirus. Genomic organization of becurtoviruses. ORFs are denoted as being encoded on the virion-sense (V) or complementary-sense (C) strand, and corresponding protein products are indicated where these are known. The position of the stem-loop containing the conserved TAAGATTCC sequence located in the large intergenic region (LIR) is shown. An intron (open box) is predicted to occur at the overlap between ORFs C1 and C2. CP, coat protein; Rep, replication-associated protein; SIR, short intergenic region.

Biology

Host range

Isolates of beet curly top Iran virus (BCTIV) have been obtained from the dicotyledonous species Beta vulgaris subsp. vulgaris (beet), Beta vulgaris subsp. maritima (sea beet), Vigna unguiculata (cowpea), Solanum lycopersicum (tomato) and Phaseolus vulgaris (common bean) (Razavinejad et al., 2013). Isolates of spinach curly top Arizona virus (SCTAV) have been found infecting the dicot species Spinacia oleracea (spinach) (Hernandez-Zepeda et al., 2013).

Transmission

The leafhopper Circulifer haematoceps was shown to transmit the virus to sugar beet under greenhouse conditions, resulting in typical curly top symptoms (Heydarnejad et al., 2013).

Species demarcation criteria

Nucleotide sequence identity: Based on the distribution of pairwise identities, a tentative species demarcation threshold of 80% is currently used (Varsani et al., 2014). Thus, pairs of becurtovirus full genome sequences with >80% pairwise identity should for now be considered as members of the same species. This criterion is tentative in that it is based on information available on only two species. As more becurtovirus sequences are discovered, this threshold may change.

Member Species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus Abbreviation(s)
Beet curly top Iran virusbeet curly top Iran virusIran-Kerman-2005/AEU273818NC_010417Complete genomeBCTIV-A[IR-Ker-05]
Beet curly top Iran virusbeet curly top Iran virusIran-Tabadkan-90-Cowpea-2010/BJX131633BCTIV-B[IR-Tab-90-cwp-10]
Beet curly top Iran virusbeet curly top Iran virusIran-Neyshabour-115-Bean-2010/CJX458087BCTIV-C[IR-Nesh-115-bea-10]
Beet curly top Iran virusbeet curly top Iran virusIran-Tabriz-8RB-Red beet-2010/DJX945572BCTIV-D[IR-Tabr-8RB-reb-10]
Spinach curly top Arizona virusspinach curly top Arizona virusUnited States-Arizona-2009HQ443515NC_015051Complete genomeSCTAV-[US-AZ-09]

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.
Download GenBank/EMBL query for sequences listed in the table here.
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