Genus: Topocuvirus


Genus: Topocuvirus

Distinguishing features

Tomato pseudo-curly top virus (TPCTV) is the only member of the genus. The virus has a monopartite genome that encodes six genes with an organization similar to that of the genomes of monopartite begomoviruses. TPCTV is the only geminivirus known to be transmitted by a treehopper (Micrutalis malleifera). The virus infects only dicots, and has been identified only in the southeastern United States (Briddon et al., 1996).

Virion 

See discussion under family properties.

Genome organization and replication

The genome of TPCTV consists of a single component of circular ssDNA of 2.8 kb. The genome, encoding six proteins, resembles that of monopartite members of the genus Begomovirus (Figure 1.Topocuvirus). Nucleotide sequence comparisons suggest that TPCTV and begomoviruses diverged after a recombination event altered insect vector specificity (Briddon et al., 1996). The coat protein (gene V1) is more closely related to those of the leafhopper-transmitted curtoviruses than to those of the whitefly-transmitted begomoviruses. The V2 protein is distantly related to the curtovirus V2 protein.

Figure 1.Topocuvirus. Genomic organization of topocuviruses. ORFs are denoted as either being encoded on the virion-sense (V) or complementary-sense (C) strand, and corresponding protein products are indicated where these are known. The position of the stem-loop containing the conserved TAATATTAC sequence located in the intergenic region (IR) is shown. CP, coat protein; Rep, replication-associated protein.

Antigenicty

The TPCTV virion is antigenically closely related to that of beet curly top virus (BTCV) in the genus Curtovirus.

Biology

Host range

The host range of TPCTV is restricted to dicotyledonous plants, and includes weed species such as nightshade (Solanum nigrum), Datura stramonium and common chickweed (Stellaria media), crops such as tomato and common bean, and the experimental host Nicotiana benthamiana. TPCTV induces symptoms resembling those associated with BCTV infection in many hosts (Briddon et al., 1996).

Transmission

TPCTV is transmitted in nature by the treehopper Micrutalis malleifera Fowler (order Hemiptera, family Membracidae), and has been transmitted experimentally to plants by Agrobacterium-mediated transfer (agroinoculation) from a tandemly repeated cloned genomic DNA (Briddon et al., 1996).

Member Species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus abbreviationIsolate abbreviation
Tomato pseudo-curly top virustomato pseudo-curly top virusUS/Florida/1994X84735NC_003825Complete genomeTPCTV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.