The genome of members of the subfamily Comovirinae consists of two ssRNAs with a 5ʹ-bound polypeptide (VPg) and a 3ʹ-poly(A) tail. Members of the subfamily group as a single branch in phylogenetic trees using the conserved Pro-Pol region (see family section on phylogenetic relationships and Figure 4.Secoviridae). Other genera are more distantly related. Within the sub-family, genera are distinguished by their specific genomic organization, biological properties and phylogenetic relations. Each genus within the sub-family Comovirinae represents a single sub-branch in the Pro-Pol phylogenetic tree.