Genus: Bymovirus


Genus: Bymovirus

Distinguishing features

Compared with other viruses in the family, members of the genus Bymovirus are distinct in having a divided (bipartite) genome and in being transmitted by the root-infecting parasite, Polymyxa graminis (Plasmodiophorales) a fungoid protist. 

Virion

Morphology

Virions are flexuous filaments of two modal lengths, 250–300 nm and 500-600 nm; both are 13 nm in width (Figure 1.Bymovirus).

Figure 1.Bymovirus. Virions of an isolate of barley yellow mosaic virus, stained with 1% PTA, pH 7.0. The bar represents 200 nm (from D. Lesemann).

Physicochemical and physical properties

Virion density in CsCl is 1.28–1.30 g cm−3

Nucleic acid

Virions contain two molecules of linear positive-sense, ssRNA. RNA1 is 7.5–8.0 kb and RNA2 is 3.5–4.0 kb; RNA makes up 5% by weight of particles. There is little sequence identity between the two RNAs except in the 5′-untranslated region.

Proteins

Virions have a single coat (capsid) protein (CP) of 28.5–33 kDa. The CP of barley yellow mosaic virus isolates has 297 aa.

Genome organization and replication

The two RNA molecules are translated initially into precursor polypeptides from which functional proteins are derived by proteolytic processing (Figure 2.Bymovirus). The organization of RNA1 is similar to that of other potyviruses but without the P1 and HC-Pro proteins. The RNA2 polyprotein is unique to bymoviruses although the first  protein encoded by RNA2 (P1, ca. 28 kDa) has aa domains with sequence similarities to the potyvirus protein HC-Pro. The larger protein of RNA2 (P2) is believed to have a role in vector transmission (You and Shirako 2010).

Figure 2.Bymovirus. Genomic map of the bymovirus bipartite genome, using, as an example an isolate of barley yellow mosaic virus. Conventions are as for potyvirus genome organization map (Figure 2.Potyviridae). Function of most mature proteins are postulated by analogy with members of the genus Potyvirus. P1 corresponds to the C-terminal protease of HC-Pro.

Antigenicity

The viral proteins are moderately immunogenic; apart from barley mild mosaic virus, most members of the genus are serologically related. The CP aa sequence identity among members is 35–74%.

Biology

Cytology

There are characteristic pinwheel-like inclusions and membranous network structures are formed in the cytoplasm of infected plant cells. No nuclear inclusions are found.

Host range

The host range of bymoviruses is narrow, restricted to the host family Gramineae. Each member has a very restricted host range; for example, the barley-infecting viruses do not infect wheat and vice versa. 

Transmission

Bymoviruses are transmitted by Polymyxa graminis in a persistent manner, surviving in resting spores as long as these remain viable; they are transmissible experimentally by mechanical inoculation, sometimes with difficulty.

Species demarcation criteria

See discussion under family description.

Member species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus abbreviationIsolate abbreviation
Barley mild mosaic virusbarley mild mosaic virusUK-FRNA1: Y10973; RNA2: X90904RNA1: NC_003483; RNA2: NC_003482Complete genomeBaMMV
Barley yellow mosaic virusbarley yellow mosaic virusYanchengRNA1: AJ132268; RNA2: AJ132269RNA1: NC_002990; RNA2: NC_002991Complete genomeBaYMV
Oat mosaic virusoat mosaic virusCranbrookRNA1: AJ306718; RNA2: AJ306719RNA1: NC_004016; RNA2: NC_004017Complete genomeOMV
Rice necrosis mosaic virusrice necrosis mosaic virusKa-1RNA1: LC055681; RNA2: LC060925RNA1: NC_028144; RNA2: NC_028145Complete genomeRNMV
Wheat spindle streak mosaic viruswheat spindle streak mosaic virusFRX73883Partial genomeWSSMV
Wheat yellow mosaic viruswheat yellow mosaic virusJARNA1: D86634; RNA2: D86635RNA1: NC_002350; RNA2: NC_002349Complete genomeWYMV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.

Derivation of names

Bymovirus: from barley yellow mosaic virus

Related, unclassified viruses

Virus name

Accession number

Virus abbreviation

Soybean leaf rugose mosaic virus

AB560671*

 SLRMV

Virus names and virus abbreviations are not official ICTV designations.
* incomplete RNA1 genome sequence