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Compared to the other genera in the Flaviviridae, pestiviruses encode two unique gene products, namely Npro and Erns (Tautz et al., 2015) The first protein of the ORF, nonstructural protein Npro, which possesses an autoproteolytic activity and is responsible for its release from the nascent polyprotein (Gottipati et al., 2014, Rumenapf et al., 1998, Stark et al., 1993), is not essential for virus replication in cell culture (Tratschin et al., 1998). One of the three viral envelope glycoproteins, Erns, possesses an intrinsic RNase activity (Krey et al., 2012, Schneider et al., 1993). Both of these unique proteins of the pestiviruses are involved in repression of the host type I IFN response (Schweizer and Peterhans 2001, Meyers et al., 2007, Ruggli et al., 2005, Ruggli et al., 2009, Python et al., 2013, Zurcher et al., 2014, Hilton et al., 2006, La Rocca et al., 2005). Two biotypes of pestiviruses, cytopathogenic (cp) and non-cytopathogenic (noncp) viruses, are distinguished by their ability to cause cytopathic effects in cell culture (Tautz et al., 2015, Becher and Tautz 2011).
Virions are 40–60 nm in diameter and spherical in shape (Figure 1.Pestivirus) (Laude 1979). The virion envelope has 10–12 nm ring-like subunits on its surface. The structure and symmetry of the core have not been characterized.
Figure 1.Pestivirus. Negative contrast electron micrograph of particles of an isolate of bovine viral diarrhea virus 1. The bar represents 100 nm. (From M. König, with permission)
Virion Mr has not been determined precisely. Buoyant density in sucrose is 1.10–1.15 g cm−3; S20,W is 140–150S (Laude 1979, Maurer et al., 2004). Virion infectivity is stable over a relatively broad pH range, but unstable at temperatures above 40 °C. Organic solvents and detergents rapidly inactivate these viruses (Depner et al., 1992).
The virion RNA is a positive sense, infectious molecule of ssRNA about 12.3-13.0 kb in size encoding a single ORF (Becher et al., 2014, Becher et al., 1998, Collett et al., 1988, Meyers et al., 1989). The 5′-NCR contains an IRES and is about 370–385 nt in length (Poole et al., 1995). The 3′-NCR, with about 185–273 nt, is complex and contains a region with variable sequences and a highly conserved terminal region (Pankraz et al., 2005, Yu et al., 1999). For some cp pestivirus strains, a small and variable segment of host cell or viral nucleic acid is integrated into particular regions (often within NS2 or directly upstream of NS3) of the viral genome, sometimes accompanied by viral gene duplications or deletions (Tautz et al., 2015, Becher and Tautz 2011). Other cp pestiviruses contain only viral gene duplications involving all or part of the Npro and NS3 to NS4B protein-coding regions, resulting in genomic RNA of up to about 16.5 kb. In all cases, the single large ORF is maintained. Finally, cp viruses may also arise by deletion of large portions of their genomes. Such defective genomes can be rescued by co-infecting intact helper viruses (Tautz et al., 2015, Becher and Tautz 2011, Abbas et al., 2013)
Virions contain four structural proteins: a basic nucleocapsid core protein, C (14 kDa) and three envelope glycoproteins, Erns (gp44/48), E1 (gp33) and E2 (gp55). All three glycoproteins exist as intermolecular disulfide-linked complexes: Erns homodimers, E1-E2 heterodimers and E2 homodimers (Rumenapf et al., 1991, Thiel et al., 1991, Weiland et al., 1992, Weiland et al., 1990). The Erns protein possesses an intrinsic RNase activity. Pestiviruses encode eight nonstructural (NS) proteins among which Npro (23 kDa), p7 (7 kDa) and NS2 (40 kDa) are not necessary for RNA replication (Behrens et al., 1998, Tautz et al., 1999). Npro is a proteinase that auto-catalytically releases itself from the nascent polyprotein. Nonstructural protein p7 is presumed to have a role in virus maturation (Harada et al., 2000, Elbers et al., 1996). NS2-3 (120 kDa) is a multifunctional protein. The N-terminal 40% (NS2) is hydrophobic and contains a zinc finger motif that binds divalent metal ions (De Moerlooze et al., 1990, Lackner et al., 2006). NS2 is a cysteine protease that is responsible for processing of NS2-3 to give rise to NS2 and NS3 (Lackner et al., 2006). NS3 (80 kDa) acts as both a serine protease involved in polyprotein processing and an RNA helicase/NTPase involved in RNA replication (Tautz et al., 1997, Tautz et al., 2000, Warrener and Collett 1995, Wiskerchen and Collett 1991). NS2-3 is found after infection with all pestiviruses. In cells infected with cp pestiviruses, large amounts of NS3 can be detected. For noncp BVDV, noncp BDV and CSFV strains, efficient NS2-3 cleavage is limited to the first eight hours of infection and at later time points the cleavage products NS2 and NS3 are difficult to detect (Lackner et al., 2004). The NS4A (7 kDa) protein acts as a cofactor to the NS3 protease activity (Tautz et al., 2000). The role of NS4B (33 kDa) is unknown. NS5A (58 kDa) represents a phosphorylated protein and also plays still to be further characterized roles in RNA replication and virion morphogenesis (Chen et al., 2012, Isken et al., 2014, Tellinghuisen et al., 2006, Xiao et al., 2009). NS5B (75 kDa) possesses RdRp activity (Steffens et al., 1999, Zhong et al., 1998).
The viruses are enveloped, but no reports have described the lipid composition.
All virus envelope glycoproteins contain N-linked glycans (Thiel et al., 1991).
The genomic RNA contains a single large ORF encoding a polyprotein of about 3900 aa that is preceded by a 5′-NCR of about 370–385 nt and followed by a 3′-NCR of about 185–273 nt. The gene order is 5′-Npro-C-Erns-E1-E2-p7-NS2-3(NS2-NS3)-NS4A-NS4B-NS5A-NS5B-3′ (Figure 2.Pestivirus) (Tautz et al., 2015, Abbas et al., 2013).
Pestivirus replication is initiated by receptor-mediated endocytosis involving more than one cell surface molecule and the viral glycoproteins Erns and E2 . CD46 has been shown to function as a cellular receptor for BVDV but is not by itself sufficient to mediate infection (Maurer et al., 2004, Krey et al., 2006, Krey et al., 2006, Krey et al., 2005). After endocytosis and uncoating, the genome RNA serves as mRNA; there are no subgenomic mRNA molecules. Translation initiation occurs by a cap-independent internal initiation mechanism involving a type IV IRES within the 5′-NCR of the RNA (Lozano and Martinez-Salas 2015). Polyprotein processing occurs co- and post-translationally by both cellular and viral proteases (Tautz et al., 2015). Nonstructural protein Npro, the first protein of the ORF, auto-proteolytically removes itself from the nascent polyprotein by cleavage at the Npro/C site. Downstream cleavages that produce structural proteins C, Erns, E1 and E2 as well as p7 are mediated by cellular signal peptide peptidase and signal peptidase(s) (Elbers et al., 1996, Bintintan and Meyers 2010, Heimann et al., 2006, Rumenapf et al., 1993). Glycoprotein translocation to the endoplasmic reticulum occurs by an internal signal sequence, within the C-terminal region of the C protein. Cleavage between E2 and p7 is not complete, leading to two intracellular forms of E2 with different C-termini (Elbers et al., 1996). Depending on the pestivirus biotype, NS2-3 either remains mostly intact or is found at reduced levels together with high amounts of its N- and C-terminal products NS2 and NS3 (Lackner et al., 2004). The increased generation of NS3 in cp pestiviruses is in most cases due to gene insertion, deletion, duplications or rearrangements (Tautz et al., 2015, Becher and Tautz 2011). The NS3/NS2-3 serine protease activity is responsible for all processing events downstream of NS3. NS4A facilitates cleavages by the NS3 protease of sites 4B/5A and 5A/5B (Tautz et al., 2015).
RNA replication probably occurs in association with intracytoplasmic membranes, presumably in a replication complex composed of viral RNA and viral nonstructural proteins. Nonstructural proteins NS3, 4A, 4B, 5A and 5B are necessary for RNA replication; only NS5A can be provided in trans (Grassmann et al., 2001). Replicative forms of viral RNA have been detected (Gong et al., 1996). The ratio of positive-to-negative sense RNA in cells 12 hours post-infection is about 10. RNA synthesis is resistant to actinomycin D. Virus maturation is poorly understood, but viral proteins are not found on the cell surface (Weiland et al., 1999), suggesting that viruses mature in intracellular vesicles and are released by exocytosis. Considerable amounts of infectious virus remain cell-associated. Host cell RNA and protein synthesis continues throughout infection.
Figure 2.Pestivirus. Pestivirus genome organization (not to scale) and polyprotein processing. The RNA is 8.9 – 11.3 kb in size, comprising a 5′-NCR of 370–385 nt, the single ORF of about 11.7 kb and the 3′-NCR of 185-273 nt. The viral nonstructural proteins are indicated as NS. P′, P″, P′″, H and R symbols indicate the localization of the Npro protease, the NS2 protease, the NS3 protease, the NS3 RNA helicase and the NS5B RdRp, respectively. The proteases and proteolytic steps involved in the generation of individual proteins are indicated. In noncp BVD viruses, NS2-3 cleavage is detectable early after infection whereas in cp BVD viruses both NS2-3 and NS3 are produced continuously.
Pestiviruses are antigenically related and cross-reactive epitopes have been documented for all species investigated (Tautz et al., 2015, Moennig et al., 1987). Separate antigenic determinants defined by monoclonal antibodies (Mabs) have also been identified. Antigenic variation is particularly pronounced among isolates of BVDV and BDV (Becher et al., 2003, Postel et al., 2015). The N-terminal portion of E2 contains an antigenically hypervariable region (van Rijn et al., 1994, van Rijn et al., 1992). Mab binding patterns are generally consistent with the genetic relatedness of viruses.
Infected animals mount potent antibody responses to two structural glycoproteins (Erns, E2) and to the NS2-3/NS3 protein, while antibody responses to other virus-encoded polypeptides are weak or not detectable. Erns and E2 are able to induce protection independently and Mabs reactive with these proteins can neutralize virus infectivity (Weiland et al., 1992, Weiland et al., 1990, Hulst et al., 1993, van Zijl et al., 1991, Reimann et al., 2004).
Pestiviruses infect pigs and ruminants, including cattle, sheep, goats and wild ruminants (Becher et al., 1997, Vilcek and Nettleton 2006). There are no invertebrate hosts. Transmission occurs by direct and indirect contact (e.g., nasal or urine secretion, faeces, contaminated food, etc.) and transplacentally. Infections may be subclinical or produce a range of clinical conditions including acute diarrhea, acute hemorrhagic syndrome, acute fatal disease, and a wasting disease. Transplacental infection can result in fetal death, congenital abnormalities, or lifelong persistent infection (Moennig and Plagemann 1992). Fatal mucosal disease can occur in cattle persistently infected with noncp viruses when a cp virus is generated by mutation or introduced by superinfection (Meyers and Thiel 1996). Pestivirus infections of livestock are economically important worldwide (Moennig and Becher 2015, Houe 2003).
Experimental infection models have not been established for bovine or ovine pestiviruses outside their natural mammalian hosts; CSFV can be adapted to propagate in rabbits (Tautz et al., 2015). Cells derived from natural host species (bovine, porcine, ovine) support virus replication. Most virus isolates are noncp and can establish persistent infections in cell culture. Infectious noncp BVDV is often present in bovine serum products used for cell culture (Buttner et al., 1997). Cp pestiviruses induce extensive cytopathology and apoptosis, and form virus plaques under appropriate conditions (Hilbe et al., 2013, Birk et al., 2008, Grummer et al., 2002, Jordan et al., 2002). No hemagglutinating activity has been found associated with pestiviruses.
Species demarcation criteria in the genus include:
Pestivirus species demarcation considers several parameters and their relationship to the type viruses of the four currently recognized species (BVDV-1 NADL; BVDV-2 890; BDV X818; and CSFV A187). Nucleotide sequence relatedness is an important criterion for pestivirus species demarcation. The complete genome sequences among the four currently recognized species and the additional related but as yet unclassified giraffe pestivirus, Pronghorn antelope virus, Bungowannah virus, atypical ruminant pestivirus (Hobi-like), sheep pestivirus (Aydin-like), rat pestivirus, and atypical porcine pestivirus (APPV) are over 25% divergent (Postel et al., 2015, Postel et al., 2016, Firth et al., 2014, Hause et al., 2015). Accordingly, at least for the currently known pestiviruses, the sequence divergence threshold of 25% for whole genome sequences provides a solid basis for pestivirus species demarcation. Convalescent animal sera generated against members of a given species (e.g., Bovine viral diarrhea virus 1) generally show a several-fold higher neutralization titre against viruses of the same species than against viruses from the other species (Becher et al., 2003, Postel et al., 2015). Finally, differences in host of origin and disease can assist in species demarcation.
For example, Bovine viral diarrhea virus 1 and Classical swine fever virus are considered different species because their members differ from each other by: (i) at least 25% at the sequence level (complete genomes), (ii) at least 10-fold difference in neutralization titre in cross-neutralization tests with polyclonal immune sera, and (iii) host range, in that under natural conditions CSFV infects only pigs while BVDV-1 infects ruminants as well as pigs.
BVDV-1 can be further subdivided into at least seventeen genotypes, while for CSFV three genotypes (CSFV-1, CSFV-2, and CSFV-3) are recognized (Yesilbag et al., 2014, Postel et al., 2012). Genotypes of these Pestivirus species can be further divided into subgroups.
In order to officially establish a novel pestivirus species, the complete genomic sequence of at least one virus isolate together with data on antigenic relatedness and host range should be considered.
More divergent pestiviruses have recently been described infecting pigs, various domestic and wild ruminant species, bats, and Norway rats and have yet to be assigned species status (Postel et al., 2015, Postel et al., 2016, Firth et al., 2014, Hause et al., 2015, Harasawa et al., 2000, Schirrmeier et al., 2004, Vilcek et al., 2005, Kirkland et al., 2007, Wu et al., 2012).
SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus abbreviationIsolate abbreviation
giraffe pestivirus H138
pronghorn antelope pestivirus
atypical ruminant pestivirus-Th/04/KhonKaen
sheep pestivirus Aydin/04/TR
atypical porcine pestivirus
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