Abbreviations : Report Help
Beetle vectors are recorded for most bromoviruses but the efficiency of such transmission is low. A long-standing report of transmission by nematodes has yet to be confirmed.
Virions are polyhedral, and all the same size, with a diameter of 27 nm.
Virions prepared below pH 6.0 have S20,w of 88S, are stable to high salt and low detergent concentrations, and are nuclease- and protease-resistant. At pH 7.0 and above, virions swell to a diameter of 31 nm, S20,w decreases to 78S, salt and detergent stability decreases dramatically, and protein and RNA are susceptible to hydrolytic enzymes. This swelling is accompanied by conformational changes of the capsid that are detectable by physical and serological means.
RNA 3′-termini are tRNA-like, are very similar in all viruses sequenced so far, and can be aminoacylated with tyrosine.
Virions contain a single coat protein (CP) of 20.3 kDa.
Lipids are not associated with virions
Carbohydrates are not associated with virions
The genome is organized as depicted in Figure 2A.Bromoviridae. Coat protein is not required for activation of the genome. Intra-segmental crossover as well as homologous recombination may occur in brome mosaic virus (BMV) RNAs. Both, viral RNA sequences and host protein contribute to BMV RNA recombination (Kolondam et al., 2015). Data from NGS revealed that BMV virions can carry host RNAs that potentially can mediate horizontal gene transfer in plants (Shrestha et al., 2018).
The natural host range is narrow, and is limited to a few plant hosts for each member of this genus. All members of the species are thought to be beetle-transmitted. BMV is also transmitted by aphids in a non-persistent manner but with poor efficiency.
All members are serologically related, with large antigenic differences between species.
Bromo: from the type species Brome mosaic virus
Criteria used for demarcation of species within the genus are host range, serological relationships, comparable replicase proteins (i.e. 1a and 2a proteins) and nucleotide sequence identity between species, which ranges from 50 to 80% depending on the gene used for comparison.
Support for preparation of the Online Report and Report Summaries has been provided by: