Genus: Limnipivirus


Genus: Limnipivirus

Distinguishing features

The genus is distinguished on the basis of genetic characters.

Virion

Morphology

No surface morphology of the icosahedral capsid is visible by EM.

Nucleic acid

Genome (Lange et al., 2014, Barbknecht et al., 2014, Phelps et al., 2014): c. 7700–8050 nt (5′-UTR: >501–712 nt; ORF: 6810–7011 nt; 3′-UTR: 312–363 nt). 5′-UTR contains a type IV IRES. The location of the cre has not been identified. 

Genome organization and replication

Genome layout:

VPg+5′-UTRIRES-IV-[1AB-1C-1D-2A1npgp/2A2npgp/-2B-2C/3A-3B-3C-3D]-3′-UTR-poly(A)

The deduced polyprotein is of 2,337 aa. There is no L protein. 1AB remains uncleaved and has a N-terminal myristoylation signal. There are two aphthovirus-like 2A proteins with NPG↓P motif.

Antigenicity

See discussion under family description.

Biology

Limnipiviruses infect freshwater fish. The virus can be grown in various fish cell lines. Experimental infection of bluegills with bluegill picornavirus induces morbidity (inflammation and redness at the base of fins, exophthalmia, abdomen distension, internal hemorrhaging and ascites) and mortality.

Species demarcation criteria

Members of a species of the genus Limnipivirus share a common genome organization.

The divergence (number of differences per site between sequences) between members of different Limnipivirus species ranges from 0.3–0.43 for P1 and 0.49–0.57 for 3CD. Sequence data indicate a second genetic type of Limnipivirus B.

Member species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus abbreviationIsolate abbreviation
Limnipivirus Abluegill picornavirus04-032JX134222NC_018506Complete genomeBGPV
Limnipivirus Bcarp picornavirusF37/06KF306267NC_023162Complete genomeCPV
Limnipivirus Cfathead minnow picornavirusfth/1/MN/USA/2010KF183915NC_039212Complete genomeFHMPV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.

Derivation of names

Limnipi-: from Greek limne, 'lake' and picornavirus.

Related, unclassified viruses

Virus name

Accession number

Virus abbreviation

Wenling bighead beaked sandfish picornavirus [XQTMC45688]

MG600092

 

Virus names and virus abbreviations are not official ICTV designations.