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Unlike other picornaviruses, kobuvirus capsids show a distinctive surface morphology when observed by electron microscopy (Fig. 1E). The crystal structure of AiV-A was recently resolved (Sabin et al., 2016)
Virions are stable at pH 3.5.
Length of genome (Yamashita et al., 1998, Yamashita et al., 2003, Smits et al., 2013, Reuter et al., 2008, Reuter et al., 2009, Otomaru et al., 2016, Pankovics et al., 2016): up to 8,280 nt (5'-UTR: up to 744 nt; ORF: 7,299-7,467 nt; 3'-UTR: 136-240 nt). The genome of Aichi virus (AiV) has a high G+C base composition (59%). The 3′-UTR differs in the three species: members of Aichivirus A have the longest 3'-UTR at 240 nt, however, the 3′-UTRs of bovine kobuvirus (BKV) and porcine kobuvirus (PKV) are shorter at 177 and 170 nt, respectively. In AiV-1, there is a 5′-proximal stem-loop involved in RNA replication and encapsidation. The IRESs of members of the Aichivirus A and Aichivirus B species are of type V, however, those of Aichivirus C (PKV) possess a type IV IRES. The cre is located in the 2A gene region of rabbit kobuvirus but has not been identified in other kobuviruses.
The deduced polyprotein has a length of c. 2,432-2,488 amino acids. There is a leader polypeptide of unknown function, and distinctive length (170-195 aa). 1AB remains uncleaved; however, a myristoylation signal (GxxxT) is present at the amino terminus of the polypeptide. The 2A protein contains an H-Box/NC motif.
Aichivirus and bovine kobuvirus have been distinguished by neutralization of infectivity and serological assays.
AiV grows in cell cultures (BSC-1, Vero). AiV is thought to be a cause of human gastroenteritis. Kobuviruses have been isolated from humans, cattle, sheep and pigs. Kobuvirus genomes have been detected in faeces of dogs, cats, mice, ferrets, goats, rabbits, bats, birds and in sewage.
Members of a species of the genus Kobuvirus:
The divergence (number of differences per site between sequences) of known Kobuvirus species ranges from 0.37-0.60 for P1 and 0.21-0.48 for 3CD.
Sixteen types have been characterized genetically by phylogenetic clustering (Aichivirus A: 6 types, Aichivirus B: 3 types, Aichivirus C: 2 types, Aichivirus D: 2 types, Aichivirus E: 1 type, Aichivirus F: 2 types).
Aichi: from the Aichi prefecture in Japan
Kobu: from Japanese kobu, 'knuckle, bump' (reference to surface structure of virus particle).
Caprine kobuvirus [12Q108]
Norway rat kobuvirus 2 [rat/NYC-D21a/USA/2013]
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