Rhabdoviridae

Rhabdoviridae

Peter J. Walker​, Juliana Freitas-Astúa, Nicolas Bejerman, Kim R. Blasdell​, Rachel Breyta, Ralf G. Dietzgen​, Anthony R. Fooks, Hideki Kondo​, Gael Kurath, Ivan V. Kuzmin, Pedro Luis Ramos-González, Mang Shi, David M. Stone​, Robert B. Tesh, Noël Tordo, Nikos Vasilakis​ and Anna E. Whitfield​

The citation for this ICTV Report chapter is the summary published as Walker et al., (2021):
ICTV Virus Taxonomy Profile: Rhabdoviridae 2021, Journal of General Virology, (in press)

Corresponding author: Peter J. Walker (peter.walker@uq.edu.au)
Edited by: Jens H. Kuhn and Stuart G. Siddell
Posted: April 2018, updated November 2019, March 2021, September 2021
PDF: ICTV_Rhabdoviridae.pdf (November 2019 version)

Summary

The family Rhabdoviridae includes three subfamilies, 40 genera and 246 species of viruses with negative-sense, single-stranded RNA genomes of approximately 10–16 kb (Table 1.Rhabdoviridae). Virions are typically enveloped with bullet-shaped or bacilliform morphology but non-enveloped filamentous virions have also been reported. The genomes are usually (but not always) single RNA molecules with partially complementary termini. Almost all rhabdovirus genomes have 5 genes encoding the structural proteins (N, P, M, G and L); however, many rhabdovirus genomes encode other proteins in additional genes or in alternative open reading frames (ORFs) within the structural protein genes. The family is ecologically diverse with members infecting plants or animals including mammals, birds, reptiles or fish. Rhabdoviruses are also detected in invertebrates, including arthropods, some of which may serve as unique hosts or may act as biological vectors for transmission to other animals or plants. Rhabdoviruses include important pathogens of humans, livestock, fish or agricultural crops. 

Table 1.Rhabdoviridae. Characteristics of members of the family Rhabdoviridae.

Characteristic

Description

Example

vesicular stomatitis Indiana virus (AF473864), species Indiana vesiculovirus, genus Vesiculovirus

Virion

Bullet-shaped or bacilliform particle 100–430 nm in length and 45–100 nm in diameter comprised of a helical nucleocapsid surrounded by a matrix layer and a lipid envelope. Some rhabdoviruses have non-enveloped filamentous virions.

Genome

Negative-sense, single-stranded RNA of 10.8–16.1 kb (unsegmented or bi-segmented).

Replication

Ribonucleoprotein (RNP) complexes containing anti-genomic RNA are generated and serve as templates for synthesis of nascent RNP complexes containing genomic RNA.

Translation

From capped and polyadenylated mRNAs transcribed processively from each gene (3′ to 5′), sometimes containing multiple ORFs.

Host Range

Vertebrates, invertebrates and plants; many vertebrate and plant rhabdoviruses are arthropod-borne.

Taxonomy

Realm Riboviria, kingdom Orthornavirae, phylum Negarnaviricota, subphylum Haploviricotina, class Monjiviricetes, order Mononegavirales; the family Rhabdoviridae includes 33 genera that are assigned to three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, Gammarhabdovirinae) and seven additional unassigned genera, together including 246 species. There are at least 150 related, unclassified rhabdoviruses.

Viruses assigned to each of the three subfamilies and 40 genera form a monophyletic clade based on phylogenetic analysis of L sequences. Viruses assigned to each genus usually have a similar genome architecture, including the number and locations of accessory genes, and also have similarities in host range, modes of transmission and/or sites of replication in the cell.

Subfamily Alpharhabdovirinae

The subfamily includes 26 genera for viruses infecting only vertebrates, only invertebrates or vertebrate hosts and arthropod vectors. Viruses assigned to this subfamily sometimes have been referred to informally as dimarhabdoviruses (dipteran and mammalian rhabdoviruses) but this term is misleading as various members may infect birds, reptiles, amphibians, non-dipteran insects, ticks, crustaceans or nematodes.

Genus Almendravirus. The viruses assigned to this genus were isolated from mosquitoes and appear to be poorly adapted (or not adapted) to replication in vertebrates. The genome of almendraviruses features an additional gene located between the G and L genes, encoding a small viroporin-like protein.

Genus Alphanemrhavirus. This genus comprises viruses that have been detected by high-throughput sequencing in parasitic nematodes (roundworms of the phylum Nematoda). The genome of alphanemrhaviruses is relatively simple, containing the five structural protein genes, but may include an additional small ORF in the M gene (Mx) overlapping the end of the M ORF. No alphanemrhaviruses have yet been isolated.

Genus Arurhavirus. Arurhaviruses have been isolated from mosquitoes and sandflies. There is evidence of infection in rodents and possibly birds. The genome features one or more genes located between the G and L genes, including a gene encoding a viroporin-like protein. An additional gene may also be present between the N and P genes.

Genus Barhavirus. Barhaviruses have been isolated from mosquitoes and infect a range of vertebrates including humans, ruminants, rodents and marsupials. There is no evidence of disease in humans. The genome is relatively simple but may include a small overlapping ORF encoding a viroporin-like protein at the end of the G gene.

Genus Caligrhavirus. Caligrhaviruses have been detected in sea lice (crustaceans in the family Caligidae) in which they appear to cause active infections. The caligrhavirus genome is relatively simple, containing the five structural protein genes, but may include an additional gene (U1) between the G and L genes. No caligrhaviruses have yet been isolated but virions have been observed by electron microscopy.

Genus Curiovirus. Curioviruses have been isolated from midges, sandflies and mosquitoes. Vertebrate hosts are largely unknown but there is evidence of infection of birds. The genome features one or more genes located between the M and G genes, and one or more genes located between the G and L genes, including a gene encoding a viroporin-like protein.

Genus Ephemerovirus. Viruses assigned to the genus have been isolated primarily from cattle, mosquitoes or midges. Some cause an acute febrile illness in bovines that is seldom fatal. The genome of ephemeroviruses features multiple genes between the G and L genes encoding accessory proteins including a non-structural class I transmembrane glycoprotein (GNS) and a viroporin (α1).

Genus Hapavirus. This genus comprises viruses that have been isolated from mosquitoes or midges and that infect birds and mammals. The genome of hapaviruses is large and complex, featuring multiple accessory genes between P and M genes, and between G and L genes, usually including a gene encoding a viroporin-like protein. 

Genus Ledantevirus. Ledanteviruses infect mammals; many have been isolated from bats or rodents and some (or all) may be transmitted by arthropods. Some have been associated with disease in humans or cattle. The genome is relatively simple but some viruses feature an additional gene between the G and L genes encoding a small protein of unknown function. 

Genus Lostrhavirus. The single virus assigned to date to the genus Lostrhavirus was detected in a tick taken from an ill human in the USA with a rash. The genome organisation is relatively simple but includes alternative ORFs of unknown importance in the N and P genes.

Genus Lyssavirus. Lyssaviruses circulate among bats (order Chiroptera) and carnivores (order Carnivora) although they may infect all warm-blooded animals causing acute encephalomyelitis with a high fatality rate (rabies). Natural transmission is via saliva, usually through a bite by an infected animal. The genome is relatively simple, containing genes that encode five structural protein but feature a long 3′-untranslated region (ψ) in the G gene; additional proteins may be expressed from alternative initiation codons in the P gene.

Genus Mousrhavirus. The single virus assigned to date to the genus Mousrhavirus has been isolated on multiple occasions from mosquitoes in Côte d’Ivoire. The genome contains only the five canonical rhabdovirus structural protein genes (N, P, M, G and L).

Genus Perhabdovirus. Perhabdoviruses infect a wide range of teleost fish. They are transmitted through contaminated water and can cause severe haemorrhagic disease. The genome is relatively simple, containing the five structural protein genes and short intergenic regions. Perhabdoviruses are phylogenetically related to but distinct from fish rhabdoviruses assigned to the genus Sprivivirus

Genus Ohlsrhavirus. Ohlsrhaviruses have been detected in culicine mosquitoes from Europe, Asia and America. Although vertebrate hosts of ohlsrhaviruses have not been identified, mosquitoes of the species from which they have been isolated are known viral vectors. Their genomes contain only the five canonical rhabdovirus structural protein genes (N, P, M, G and L).

Genus Sawgrhavirus. Sawgrhaviruses have been isolated from hard ticks (Ixodidae) collected in North America. Their genomes contain the five canonical rhabdovirus structural protein genes (N, P, M, G and L); alternative ORFs of unknown importance are conserved in the G genes of some sawgrhaviruses.

Genus Sigmavirus. Sigmaviruses are transmitted vertically, each virus infecting a fly of a single species in the families Drosophilidae or Muscidae. Infection results in paralysis or death of flies upon exposure to carbon dioxide. The genome may feature an additional gene (X) located between the M and G genes, encoding a protein of unknown function.

Genus Sprivivirus. The viruses assigned to this genus infect a wide range of teleost fish. They are transmitted through contaminated water and can cause severe haemorrhagic disease. The genome of spriviviruses is relatively simple, containing the five structural protein genes and short intergenic regions. Spriviviruses are phylogenetically related to but distinct from fish rhabdoviruses assigned to the genus Perhabdovirus

Genus Sripuvirus. Viruses assigned to this genus have been isolated from either sandflies or lizards.  The genome of sripuviruses features a small protein encoded in a consecutive ORF in the M gene and a small transmembrane protein encoded in an alternative ORF at the start of the G gene.

Genus Sunrhavirus. Sunrhaviruses have been isolated from culicine mosquitoes, biting midges and birds in Africa, Australia and South America. Their genome features an additional gene between the M and G genes encoding a small hydrophobic protein and alternative ORFs in several genes including, consistently, the P gene.

Genus Tibrovirus. Some tibroviruses infect cattle and water buffalo and are transmitted by midges; several other tibroviruses have been detected in humans but their role in human disease is currently unclear. The genome features two accessory genes between the M and G genes, and a gene encoding a viroporin-like protein between the G and L genes.

Genus Tupavirus. Tupaviruses have been isolated from birds, insectivores and rodents, and there is evidence of infection in other vertebrates. The genome features a long alternative ORF in the P gene and an additional gene encoding a small hydrophobic protein between the M and G genes.

Genus Vesiculovirus. Vesiculoviruses infect a wide range of vertebrate hosts and are transmitted by insects; some may also be transmitted amongst vertebrates by direct contact. Several vesiculoviruses cause vesicular stomatitis in livestock and/or have been associated with influenza-like illness and encephalitis in humans. The genome is relatively simple, containing the five structural protein genes and short intergenic regions, but may also include alternative ORFs in the P gene and use of alternative initiation codons in the M gene.

Genus Zarhavirus. The single virus assigned to date to the genus Zarhavirus was isolated from hard ticks collected in Iran. The genome contains only the five canonical rhabdovirus structural protein genes (N, P, M, G and L).

Subfamily Betarhabdovirinae

The subfamily includes six genera for viruses infecting plant hosts and arthropod vectors.

Genus Alphanucleorhabdovirus. Alphanucleorhabdoviruses infect a wide range of monocot and dicot plants and are transmitted by arthropod vectors (planthoppers, leafhoppers) in which they replicate. Alphanucleorhabdoviruses have unsegmented genomes and replicate in the nuclei of infected plant cells. Alphanucleorhabdoviruses form a monophyletic clade within a larger cluster containing the betanucleorhabdoviruses, gammanucleorhabdoviruses, and dichorhaviruses. They feature an additional gene between the P gene and M gene encoding a movement protein.

Genus Betanucleorhabdovirus. Betanucleorhabdoviruses infect a wide range of dicot plants and some members are transmitted by aphids in which they replicate. Betanucleorhabdoviruses have unsegmented genomes and replicate in the nuclei of infected plant cells. Betanucleorhabdoviruses form a monophyletic clade, being a sister clade to dichorhaviruses. They feature an additional gene between the P gene and M gene encoding a movement protein.

Genus Cytorhabdovirus. Viruses assigned to this genus infect a wide range of plants and are transmitted by arthropod vectors (aphids, planthoppers, leafhoppers or whiteflies) in which they replicate. In plant cells, cytorhabdoviruses replicate in the cytoplasm. Cytorhabdoviruses have an unsegmented genome featuring an additional gene located between the P gene and M gene, encoding a movement protein; some may also encode a viroporin-like protein.

Genus Dichorhavirus. Dichorhaviruses infect plants and are transmitted by Brevipalpus mites. They cause localised lesions on leaves, stems, and fruits of economically important plants such as citrus, coffee and orchids. The genome of dichorhaviruses is bi-segmented: RNA1 contains the N, P, M and G genes, and an additional gene located between the P gene and M gene encoding a putative movement protein; RNA2 contains the L gene. Virions formed in plant cells may lack envelopes.

Genus Gammanucleorhabdovirus. This genus currently includes only one species with a single virus, maize fine streak virus (MFSV), that infects maize and is transmitted by leafhoppers in which it replicates. The MFSV genome features two additional genes between the P gene and M gene, one of them encoding a movement protein. MFSV has an unsegmented genome and replicates in the nuclei of infected plant cells. MFSV is phylogenetically closest to alphanucleorhabdoviruses, betanucleorhabdoviruses, and dichorhaviruses but with weak bootstrap support; sequence similarities with other plant rhabdoviruses are low.

Genus Varicosavirus. The varicosavirus lettuce big vein-associated virus (LBVaV) is transmitted in soil and zoospores of a chytrid fungus, Olpidium brassicae. The genome of varicosaviruses is bi-segmented: RNA1 encodes the L protein and, in some members, contains a small ORF preceding the L gene; RNA2 contains 3 to 5 ORFs including the coat protein gene. LBVaV virions observed in plant cells are non-enveloped rods resembling intracellular nucleocapsids of other rhabdoviruses.

Subfamily Gammarhabdovirinae

The subfamily includes only a single genus for viruses infecting finfish. Viruses assigned to this subfamily are very distant phylogenetically from fish rhabdoviruses assigned to the genera Perhabdovirus and Sprivivirus (subfamily Alpharhabdovirinae).

Genus Novirhabdovirus. Novirhabdoviruses infect teleost fish of numerous species in which they can cause severe haemorrhagic disease. Transmission is waterborne; there is also evidence for egg-associated transmission. The genome features an additional gene (NV) that is located between the G and L genes. The NV protein appears to be involved in evasion of the host interferon response.

Genera not assigned to a subfamily

Within the Rhabdoviridae, seven genera do not fall within any of the three existing subfamilies.  All viruses assigned to these genera have been detected by high-throughput sequencing of metagenomes of invertebrates.

Genus Alphacrustrhavirus. The viruses in this genus have been detected in marine crustaceans (order Decapoda).

Genus Alphadrosrhavirus. The viruses in this genus have been detected in flies of various species (Diptera: Drosophilidae). The genome features an additional gene between the G and L genes in which there are two overlapping ORFs, each of which encodes a small hydrophobic protein with a strongly predicted transmembrane domain.

Genus Alphahymrhavirus.  The viruses in this genus have been detected in ants and wasps (Hymenoptera) and are distinct phylogenetically from rhabdoviruses assigned to the genus Betahymrhavirus.

Genus Betahymrhavirus. The viruses in this genus have been detected in wasps (Hymenoptera) and are distinct phylogenetically from rhabdoviruses assigned to the genus Alphahymrhavirus. The genome features an additional gene between the M gene and G gene with two overlapping reading frames and a “slippery” sequence in the overlap region that could allow expression of the second ORF by ribosomal frame-shift.

Genus Betanemrhavirus. The viruses in this genus have been detected in parasitic roundworms (Ascaridida: Ascarididae). The genome features an additional gene between the P and M genes.

Genus Betapaprhavirus. The viruses in this genus have been detected in moths and butterflies (Lepidoptera). The genome features an additional gene between the G and L genes encoding a small basic protein.

Genus Betaricinrhavirus. The viruses in this genus have been detected in hard ticks (Acari: Ixodidae).  The genome features an alternative ORF in the N gene, overlapping the end of the N ORF.

Virion

Morphology

Enveloped virions have been reported to be in the range of 100–460 nm in length and 45–100 nm in diameter (Hummeler et al., 1967, Hummeler and Koprowski 1969, Nakai and Howatson 1968, Knudson 1973, Francki 1973) (Figure 1.Rhabdoviridae). The longer forms may represent virions fused end-to-end. Defective-interfering (DI) virus particles are proportionally shorter (Huang et al., 1966). Viruses infecting vertebrates are typically bullet-shaped or cone-shaped; however, some rhabdoviruses infecting animals as well as unsegmented plant rhabdoviruses appear bacilliform when fixed prior to staining (Vasilakis et al., 2013, Kurz et al., 1986). In unfixed preparations, they may appear bullet-shaped or pleomorphic. The outer surface of virions (except for the quasi-planar end of bullet-shaped viruses) is covered with projections (peplomers) which are 5–10 nm long and about 3 nm in diameter (Hummeler et al., 1967). They consist of trimers of the viral envelope glycoprotein (G). A honeycomb pattern of peplomers is observed on the surface of some viruses. Internally, the nucleocapsid (30–70 nm in diameter) has helical symmetry and appears to have cross-striations (spacing 4.5–5 nm) in negatively-stained and thin-sectioned virions (Nakai and Howatson 1968, Cartwright et al., 1972, Simpson and Hauser 1966). The nucleocapsid consists of a ribonucleoprotein (RNP) complex comprising the genomic RNA and tightly bound nucleoprotein (N) together with an RNA-directed RNA polymerase (L) and polymerase-associated phosphoprotein (P). The RNP complex is active for transcription and replication: the N-RNA template is processed by L, which contains various enzymatic activities, and its cofactor P (Emerson and Wagner 1972, Emerson and Yu 1975). In the cytoplasm, the RNP complex is uncoiled and filamentous, about 700 nm in length and 20 nm in diameter (Sokol et al., 1969). In the virion, the lipid envelope containing G interacts with the coiled RNP complex via the matrix protein (M). Virions reported for some plant rhabdoviruses appear to lack a viral envelope (Dietzgen et al., 2014).

 

Figure 1.Rhabdoviridae. (A) Negative-contrast electron micrograph of vesicular stomatitis Indiana virus particles. The bar represents 100 nm (Courtesy of P. Perrin). (B) Negative-contrast electron micrograph of rabies virus defective-interfering (DI) particles. (Courtesy of P. Perrin). (C) Schematic illustration of a rhabdovirus virion and ribonucleocapsid structure. Unravelling of the RNP is illustrative only to show more clearly its association with the L and P proteins (Courtesy of P. Le Mercier, ViralZone, SIB Swiss Institute of Bioinformatics). 

Physicochemical and physical properties

Reported virion Mr ranges from 0.3–1.0 x 109 and the S20w is in the range 550­–1045 S (plant rhabdoviruses usually have larger S20w values) (Neurath et al., 1966, Jackson and Christie 1977). Virion buoyant density is 1.19–1.20 g cm−3 in CsCl and 1.16–1.19 g cm−3 in sucrose (Jackson and Christie 1977, McCombs et al., 1966, Warrington 1965, Sokol et al., 1968). Virus infectivity is rapidly inactivated at 56 °C, or following UV-, gamma- or X-irradiation, or exposure to formalin or lipid solvents such as detergents (Olitsky and Long 1928, Shechmeister et al., 1962).

Nucleic acid

Virions typically contain a single molecule of linear, negative-sense single-stranded RNA (Mr 3.4 x 106 to 5.4 x106; approximately 10–16 kb); rhabdoviruses with segmented genomes also may occur with each RNA segment encapsidated independently (Kormelink et al., 2011, Sasaya et al., 2001). The RNA typically represents about 1–3% of virion weight (Knudson 1973, McSharry and Wagner 1971, Thomas et al., 1985). The RNA has a 3′-terminal free hydroxyl group and a 5′-triphosphate and is not polyadenylated (Moyer et al., 1975, Ehrenfeld and Summers 1972). The ends have inverted complementary sequences encoding transcription and replication initiation signals (Keene et al., 1979, Li and Pattnaik 1997, Whelan and Wertz 1999). Defective-interfering RNAs, usually substantially shorter than full-length RNA (less than half length), may be identified in RNA recovered from virus populations (Brown et al., 1967). They are usually negative-sense; however, hairpin RNA forms are also found. Defective-interfering RNAs replicate only in the presence of homologous and, occasionally, certain heterologous helper rhabdoviruses which provide the functional genes (Perrault 1981, Perrault and Semler 1979). Full-length positive-sense RNA, which is an intermediate during the replication process, may constitute a significant proportion of a viral RNA population (Soria et al., 1974). Like the full-length negative-sense RNA genome, the anti-genome is tightly bound to N and does not occur as naked RNA.

Proteins

Virions generally have five structural proteins (designated N, P, M, G and L; see Table 2.Rhabdoviridae for a summary of their locations, masses and functions). The structural proteins represent 65–75% of dry weight of the virion (Knudson 1973, McSharry and Wagner 1971, Thomas et al., 1985). The function(s) of each of these proteins have been determined largely from studies of the model rhabdoviruses, vesicular stomatitis Indiana virus (VSIV) and/or rabies virus (RABV); the same functions are typically assumed to apply to other rhabdoviruses, although this is not often confirmed experimentally. Most rhabdoviruses also encode multiple additional (accessory) proteins but few of the encoded proteins have been characterised. Ephemeroviruses express a class 1a viroporin (α1) and proteins with viroporin-like structures occur commonly in animal rhabdoviruses (Joubert et al., 2014, Walker et al., 2015) and plant cytorhabdoviruses. Ephemeroviruses and some hapaviruses also express large non-structural class I transmembrane glycoproteins (GNS) that are related to the envelope glycoprotein (G) and appear to have arisen by gene duplication (Walker et al., 1992, Wang and Walker 1993, Gubala et al., 2010). Novirhabdoviruses infecting fish express a non-structural protein (NV) that appears to be required for efficient replication and plays a role in evading the host innate anti-viral response (Kurath and Leong 1985, Biacchesi 2011). Plant-adapted viruses have one or more additional non-structural proteins, one of which has been shown to facilitate virus movement between plant cells (Jackson et al., 2005b). Vesiculovirus express two small proteins (C and C′) from an alternative ORF in the P gene (Spiropoulou and Nichol 1993, Peluso et al., 1996); in lyssaviruses, variant forms of P are expressed from alternative initiation codons in the same frame and are involved in modulating the interferon response (Moseley et al., 2007, Chenik et al., 1995).

For certain rhabdoviruses, other nomenclature has previously been used for P (NS, M1 or M2) and M (M1 or M2). The large number and diversity of accessory proteins encoded in rhabdovirus genomes have presented challenges for nomenclature. Some well-described accessory proteins have established names that are in common use. However, as the amino acid sequences of most accessory proteins are not highly conserved and their functions are largely unknown, a universal system of nomenclature based on genome location rather than structural or functional homology has been proposed (Walker et al., 2015). According to this system: i) each additional transcriptional unit (other than N, P, M, G and L) is designated U (unknown) followed by a number in the order they appear in the genome in positive polarity (i.e., U1, U2, U3, etc); ii) the first ORF within each transcriptional unit is assigned the same designation as the transcriptional unit; and iii) each subsequent ORF (alternative, overlapping or consecutive) within any transcriptional unit is designated with a letter (i.e., U1x, U1y, U1z). Alternative ORFs are defined as those which occur within the frame of a longer ORF; overlapping ORFs are alternative ORFs that extend beyond the frame of the primary ORF; and consecutive ORFs are those which do not overlap but follow consecutively within the same transcriptional unit. The VSIV C and C′ proteins (55 and 65 amino acids, respectively) are the smallest rhabdovirus proteins known to be expressed in infected cells (Spiropoulou and Nichol 1993, Peluso et al., 1996) and so ORFs ≥ 180 nucleotides may be considered as potentially significant, depending on their location in the transcriptional unit, the Kozak context of the initiation codon and their conservation in multiple virus isolates or related rhabdoviruses (Walker et al., 2015). 

Table 2.Rhabdoviridae. Location and functions of rhabdovirus structural proteins.

Protein

Location, mass and function

L

A component of the viral nucleocapsid (ca. 220–240 kDa) responsible for most of the functions required for transcription and replication: RdRP, mRNA 5′-capping, 3′-poly(A) synthesis and protein kinase activities. Observed masses by SDS-PAGE are 150–240 kDa.

G

Associates into trimers to form the virus surface peplomers (monomer ca. 65–90 kDa). Binds to host cell receptor(s), induces virus endocytosis then mediates fusion of viral and endosomal membranes. G is variously N-glycosylated and palmitoylated; it lacks O-linked glycans and may have hemagglutinin activity. Induces and binds virus-neutralizing antibodies and elicits cell-mediated immune responses. In some cases, G is involved in tropism and pathogenicity.

N

Major component of the viral nucleocapsid (ca. 47–62 kDa). It associates with full-length negative- and positive-sense genomic RNAs, or defective-interfering RNAs, but not mRNAs. N is an active element of the template, presenting the bases to the polymerase. Newly synthesised N probably modulates the balance between genome transcription and replication by influencing the recognition of the transcription signals. N elicits cell-mediated immune responses and humoral antibodies. In some plant rhabdoviruses, N translocates to a sub-nuclear compartment when co-expressed with the cognate P.

P

A cofactor of the viral polymerase (ca. 20–30 kDa). It is variously phosphorylated and generally migrates by SDS-PAGE as a protein of about 40–50 kDa, sometimes faster. P is essential for at least two fundamental functions: (i) it mediates the physical link and the correct positioning of L on the N-RNA template; and (ii) it acts as a chaperone during the synthesis of N, by forming N-P complexes that prevent N from self-aggregation and binding to cellular RNA. During the genome replication process, N is then transferred from these N-P complexes to the nascent viral RNA to ensure its specific encapsidation into new RNPs. P elicits cell-mediated immune responses. In several rhabdoviruses P also plays a fundamental role in evading the host innate anti-viral response.

M

A basic protein that is an inner component of the virion (ca. 20–30 kDa). It is believed to regulate genome RNA transcription. M binds to nucleocapsids and the cytoplasmic domain of G, thereby facilitating the process of budding. It is sometimes phosphorylated or palmitoylated. M is found in the nucleus and inhibits host cell transcription. It also mediates other pathological effects (cell rounding for VSIV, apoptosis for RABV, intracellular accumulation of the inner nuclear membrane for potato yellow dwarf virus (PYDV).

Lipids

Virions are composed of about 15–25% lipid, with their composition reflecting that of the host cell membrane where virions bud (Knudson 1973, McSharry and Wagner 1971, Thomas et al., 1985). Generally, phospholipids represent about 55–60%, and sterols and glycolipids about 35–40% of the total lipids. G may have covalently associated fatty acids proximal to the lipid envelope (Santiana et al., 2018, Gaudin et al., 1991).

Carbohydrates

Virions are composed of about 3% carbohydrate by weight (Knudson 1973, McSharry and Wagner 1971, Thomas et al., 1985). The carbohydrates are present as N-linked glycan chains on G and as glycolipids. Ephemerovirus GNS is also N-glycosylated (Walker et al., 1991). In mammalian cells, the oligosaccharide chains are generally of the complex type; in insect cells they are of non-complex types (Jarvis and Finn 1995). The number and location of N-glycosylation sites vary for G of different rhabdoviruses.

Genome organisation and replication

Rhabdovirus genomes contain at least five ORFs in the negative-sense genome in the order 3′-N-P-M-G-L-5′ (Kuzmin et al., 2009, Walker et al., 2011). The genes are flanked by conserved transcription initiation and termination/polyadenylation signals, about 10 nt in length. For many rhabdoviruses, additional genes are interposed between the structural protein genes and alternative, overlapping or consecutive ORFs may occur within the structural protein genes or in the additional genes. Some rhabdovirus genomes are segmented. Consequently, genomes of viruses assigned to different genera may vary greatly in length and organisation (Figure 2.Rhabdoviridae).

Figure 2.Rhabdoviridae. Schematic representation of rhabdovirus genome organizations exemplifying variations in the number and location of accessory genes. A typical member of each genus is represented. Each arrow indicates the position of a long open reading frame (ORF) as described in individual genus sections. ORFs for viroporin-like proteins are coloured yellow and those for movement proteins in blue. Other alternative ORFs occur in some genes; only ORFs (≥180 nt) that appear likely to be expressed are shown.

Most understanding of rhabdovirus replication and transcription has been obtained from studies of vesiculoviruses and lyssaviruses (Banerjee 1987, Finke and Conzelmann 2005, Banerjee and Barik 1992). Genes are transcribed sequentially (from the template virus RNA and in decreasing molar abundance) as 5′-capped, 3′-polyadenylated, monocistronic mRNAs (Moyer et al., 1975, Ehrenfeld and Summers 1972, Abraham et al., 1975, Abraham and Banerjee 1976, Ehrenfeld 1974, Banerjee and Rhodes 1973, Naito and Ishihama 1976) (Figure 3.Rhabdoviridae). A short uncapped, non-polyadenylated and untranslated leader RNA, corresponding to the complement of the 3′-terminus of the viral RNA (i.e., preceding the N mRNA), is also transcribed (Colonno and Banerjee 1976, Colonno and Banerjee 1978). Unlike mRNAs, leader RNA has a 5′-triphosphate terminus (Figure 3.Rhabdoviridae). Leader RNA of some viruses has been identified in the nucleus of infected cells. The mRNAs generally have common 5′-terminal sequences corresponding to the cap structure fused to the first nucleotides copied from the transcription initiation signal. The mRNAs also each contain a 3′-poly(A) tail which is produced by the viral transcriptase upon copying in a reiterative mode at uridine residues present in each transcription termination signal (Naito and Ishihama 1976). Very long 3′-untranslated regions (up to 750 nt) occur in some mRNAs (e.g., lyssaviruses, ephemeroviruses and hapaviruses) (Walker et al., 2015). Intergenic sequences are generally short but may be up to about 100 nt in length. In some cases, the transcription initiation signal of one gene overlaps the 3′-end of the preceding gene.

 

Figure 3.Rhabdoviridae. Genome organization, transcription and replication of vesicular stomatitis Indiana virus. Top: genome structure Middle: process of consecutive transcription of leader RNA and messenger RNAs. The role of N (green circles), P (red blob) and L (grey oval) is indicated. Bottom: replication of the negative-sense genome (light green N) via a positive-sense anti-genome intermediate (dark green N). The switch from transcription to replication is regulated by N. The genome and anti-genome strands are not generated in equimolar amounts.

Non-canonical mechanisms of translation from overlapping or consecutive ORFs appear to occur commonly in viruses assigned to some genera. Although not yet demonstrated experimentally, the likely mechanisms include: i) leaky ribosomal scanning; ii) a stop-start mechanism involving overlapping or consecutive termination and initiation codons and a ‘termination upstream ribosome-binding site’ (TURBS); and iii) ribosomal frame shifts featuring a ‘slippery’ sequence followed by a predicted pseudoknot structure (Walker et al., 2015). In the case of some rhabdoviruses, polycistronic mRNAs result from the read-through of the transcription termination signal, allowing transcription extension across the adjacent 5′-gene. However, in most cases, this appears to be due to corruption of the transcription termination signal during adaptation to growth in cell culture. 

Except for plant rhabdoviruses, which generally penetrate plant cells through mechanical damage caused by arthropod or chytrid vectors, rhabdovirus adsorption is mediated by G attachment to cell surface receptors, and penetration of the cell occurs by endocytosis via coated pits (Regan and Whittaker 2013, Albertini et al., 2012). Various candidate receptors have been postulated for RABV (nicotinic acetylcholine receptor AChR, neural cell adhesion molecule NCAM, low affinity nerve growth factor receptor p75NTR), VSIV (phosphatidyl serine), viral hemorrhagic septicemia virus (VHSV) (fibronectin), and others (Lafon 2005, Schlegel et al., 1983, Bearzotti et al., 1999). In addition, carbohydrate moieties, phospholipids and gangliosides may play a complementary role for virus binding (Coil and Miller 2004, Superti et al., 1986, Superti et al., 1984). After penetration by endocytosis, low pH within the endosome triggers fusion between endosomal and viral membranes, liberating the RNP complex into the cytoplasm. The pH-induced fusion depends on conformational changes of the glycoprotein, a process that is reversible upon raising the pH (Roche et al., 2008, Roche and Gaudin 2004). Once the nucleocapsid is released into the cytoplasm, the RNA genome is repetitively transcribed (primary transcription) by the virion transcriptase (Banerjee 1987). N removal does not occur since the transcriptase only recognizes the RNA-N protein complex as template (Arnheiter et al., 1985). The capped and polyadenylated mRNAs are generally translated in cytoplasmic polysomes, except for the G mRNA which is translated on membrane-bound polysomes (Both et al., 1975, Grubman et al., 1975, Morrison and Lodish 1975). Transcription occurs in the presence of protein synthesis inhibitors, indicating that it does not depend on de novo host protein synthesis (Villarreal and Holland 1974, Marcus et al., 1971). Following translation, RNA replication occurs in the cytoplasm (full-length positive-sense and then full-length negative-sense RNA synthesis).

Nucleorhabdoviruses (genera Alphanucleorhabdovirus, Betanucleorhabdovirus, Gammanucleorhabdovirus) and dichorhaviruses replicate in viroplasms in the cell nucleus (van Beek et al., 1985, Redinbaugh et al., 2002, Jackson et al., 2005a, Kondo et al., 2013, Kitajima et al., 2001). Replication again occurs on the RNA-N protein complex and requires the newly synthesised N, P and L species to concomitantly encapsidate the nascent RNA into a nucleocapsid structure. Apart from freshly translated N, P and L, replication may require host factors. Vesiculoviruses can replicate in enucleated cells, indicating that newly synthesised host gene products are not required (Follett et al., 1974, Wiktor and Koprowski 1974). However, as for some other negative-sense RNA viruses, trafficking of rabies virus proteins to and from the nucleus appears to play an important role in pathogenesis and modulating the host immune response to infection (Audsley et al., 2016, Wiltzer et al., 2012).

It has been proposed that the concomitant binding of N to the nascent positive- or negative-sense viral RNA species may promote replication rather than transcription, by favouring read-through of transcription termination signals (Arnheiter et al., 1985, Blumberg et al., 1981). Replication leads to the synthesis of a full-length positive-sense anti-genome RNA. This, in turn, serves as a replicative intermediate for the synthesis of negative-sense genome RNA for the progeny virions. Following replication, further rounds of transcription (secondary transcription), translation and replication ensue. A typical feature of negative-sense RNA viruses (shared by all members of the order Mononegavirales) is that the RNA genome (or anti-genome) is never “naked” in the cell but is always encapsidated by the nucleoprotein. This RNA-N complex is the true template recognised by the viral polymerase (transcriptase or replicase) (Emerson and Wagner 1972, Moyer et al., 1991). 

Post-translational trafficking and modification of G involve translocation across the endoplasmic reticulum membrane, removal of the amino-proximal signal sequence and step-wise glycosylation in compartments of the Golgi apparatus (Rothman and Lodish 1977, Zilberstein et al., 1980). Depending on the cell, G may move to the plasma membrane, particularly to the basolateral surfaces of polarised cells (Fuller et al., 1984, Pfeiffer et al., 1985). 

Viral nucleocapsid structures are assembled in association with M and lipid envelopes containing viral G to form virions (Mebatsion et al., 1999). The site of formation of particles depends on the virus and host cell. For vesiculoviruses, lyssaviruses, ephemeroviruses and novirhabdoviruses, nucleocapsids are synthesised in the cytoplasm and virus particles bud from the plasma membrane in most, but not all cells. Some lyssaviruses produce particles that bud predominantly from intracytoplasmic membranes and in some cases prominent virus-specific cytoplasmic inclusion bodies containing N are observed in infected cells (RABV inclusion bodies are called Negri bodies) (Manghani et al., 1986, Matsumoto 1962, Matsumoto et al., 1974, Lahaye et al., 2009). Cytorhabdoviruses bud from intracytoplasmic membranes associated with viroplasms; none have been observed to bud from plasma membranes (Jackson et al., 2005a). Nucleorhabdoviruses and dichorhaviruses bud from the inner nuclear membrane and accumulate in the perinuclear space (van Beek et al., 1985, Redinbaugh et al., 2002, Jackson et al., 2005a).

Depending on the virus and host cell type, rhabdovirus infections may inhibit cellular protein synthesis and cause apoptosis by mechanisms that are mediated by M (Koyama 1995, Kopecky et al., 2001, Weck and Wagner 1979, Jackson and Rossiter 1997, Larrous et al., 2010, Faria et al., 2005). Complementation between viral mutants of related viruses may occur (e.g., between vesiculoviruses), but not between viruses assigned to different genera (Pringle et al., 1971, Repik et al., 1976). Complementation has also been reported to occur by re-utilisation of the structural components of UV-irradiated virus (VSIV). Inter-molecular genetic recombination between different virus isolates is very rare, but intra-molecular recombination may occur during the formation of defective-interfering RNAs. Phenotypic mixing occurs between some animal rhabdoviruses and other enveloped animal viruses (e.g., paramyxoviruses, orthomyxoviruses, retroviruses, herpesviruses).

Biology

Rhabdoviruses are ecologically diverse with members infecting plants or animals including mammals, birds, reptiles or fish (Kuzmin et al., 2009, Dietzgen and Kuzmin 2012, Fu 2005, Kuzmin and Walker 2016). Some of the vertebrate rhabdoviruses have a wide experimental host range; rhabdoviruses infecting plants usually have a narrow host range among higher plants. Rhabdoviruses are also detected in invertebrates, including many arthropods, some of which may serve as biological vectors for transmission to animals or plants. A diverse range of vertebrate and invertebrate cell lines are susceptible to vertebrate rhabdoviruses in vitro

Rhabdoviruses are not usually transmitted vertically in vertebrates or plants, but transovarial transmission has been documented in insects. Sigmaviruses were recognised first as a congenital infection in fruit flies. Vector transmission may involve mosquitoes, sandflies, midges, aphids, whiteflies, leafhoppers, planthoppers or mites. Some viruses are transmitted mechanically in sap or from the body fluids of infected hosts. Mechanical transmission of viruses infecting vertebrates may be by contact, aerosol, bite, or venereal. Fish rhabdoviruses can be transmitted by exposure to contaminated water.

Antigenicity

G induces virus-neutralising antibodies which define viruses as serotypes and can provide protective immunity. Antigenic cross-reactions in complement-fixation or indirect immunofluorescence tests occur primarily between rhabdoviruses within a genus and involve antigenic determinants located on the N protein. Cross-reactions in indirect immunofluorescence tests have also been detected between some animal rhabdoviruses that are now assigned to different genera (Calisher et al., 1989, Tesh et al., 1983).

Derivation of names

Almendravirus: from Puerto Almendra, near Iquitos in northern Peru from where Puerto Almendras virus, a member of the species Puerto Almendras almendravirus, was first isolated.

Alphacrustrhavirus: from the alpha group of crustacean rhabdoviruses.

Alphadrosrhavirus: from the alpha group of drosophila rhabdoviruses.

Alphahymrhavirus: from the alpha group of hymenopteran rhabdoviruses.

Alphanemrhavirus: from the alpha group of nematode rhabdoviruses.

Alphanucleorhabdovirusfrom the alpha group of rhabdoviruses with their nuclear (nucleo: from Latin nux, nucis, “nut”) localisation of virus replication complexes.

Alphapaprhavirus: from the alpha group of lepidopteran (papilionem: from Latin “butterfly”) rhabdoviruses.

Alpharicinrhavirus: from the alpha group of tick (ricinus: from Latin “tick”) rhabdoviruses.

Arurhavirus: derived from Aruac, an ancient native tribe of Trinidadian Americans after which Aruac virus was named, and rhabdovirus.

Betahymrhavirus: from the beta group of hymenopteran rhabdoviruses.

Betanemrhavirus: from the beta group of nematode rhabdoviruses.

Betanucleorhabdovirus: from the beta group of rhabdoviruses with their nuclear (nucleo: from Latin nux, nucis, “nut”) localisation of virus replication complexes.

Betapaprhavirus: from the beta group of lepidopteran (papilionem: from Latin “butterfly”) rhabdoviruses.

Betaricinrhavirus: from the beta group of tick (ricinus: from Latin “tick”) rhabdoviruses.

Barhavirus: from Bahia Grande, a body of water near Brownsville, Texas, where Bahia Grande virus was first isolated, and rhabdovirus.

Caligrhavirus: from Caligidae, the family of copepods that includes sea lice, and rhabdovirus.

Curiovirus: from Curionopolis, a municipality near Serra Norte in northern Brazil from where Curionopolis virus, a member of the species Curionopolis curiovirus, was first isolated.

Cytorhabdovirus: from the cytoplasmic localisation of virus replication complexes (cyto: from Greek kytos, “cell”).

Dichorhavirus: from the bi-segmented characteristic of the viral genomes (Dicho: from Greek, meaning “in two, apart or asunder”).

Ephemerovirus: from the virus bovine ephemeral fever virus, assigned to the species Bovine fever ephemerovirus.

Gammanucleorhabdovirus: from the gamma group of rhabdoviruses with their nuclear (nucleo: from Latin nux, nucis, “nut”) localisation of virus replication complexes.

Hapavirus: from Hart Park serogroup which is the well-established antigenic designation of Flanders virus, Hart Park virus and several other members of the genus.

Ledantevirus: from the name of the medical centre (Hôpital Aristide Le Dantec) in Dakar, Senegal, where the patient from which Le Dantec virus, a member of the species Le Dantec ledantevirus, was first isolated. The medical centre derives its name from the French military doctor, Aristide Auguste Le Dantec, who established the first medical school in Senegal.

Lostrhavirus: from lone star tick (Amblyomma americanum) in which lone star tick rhabdovirus was first detected, and rhabdovirus.

Lyssavirus: from Lyssa, the Greek goddess of madness, rage, and frenzy, reflecting the neurological symptoms of rabies virus infection.

Merhavirus: from Merida, the capital of the Mexican state of Yucatan where Merida virus, a member of the species Merida merhavirus, was first isolated, and rhabdovirus.

Mousrhavirus: derived from Moussa (a coffee plantation in Côte d’Ivoire) where Moussa virus was first isolated, and rhabdovirus.

Novirhabdovirus: from the additional gene (NV), common to members of the genus and encoding a unique non-virion protein.

Ohlsrhavirus: from Ohlsdorf in Germany, where Ohlsdorf virus was first detected in mosquitoes, and rhabdovirus.

Perhabdovirus: from perch rhabdovirus, a member of the species Perch perhabdovirus.

Rhabdoviridae: from rhabdo(Greek) meaning rod, referring to virion morphology.

Sawgrhavirus: from Sawgrass Lake in Florida where Sawgrass virus was first isolated, and rhabdovirus.

Sigmavirus: from the virus discovered in fruit flies (Drosophila melanogaster) that was named virus “sigma” (L'Heritier 1958).

Sprivivirus: from spring viraemia of carp virus, a member of the species Carp sprivivirus.

Sripuvirus: from Sripur, the location in north-eastern Bangladesh from where Sripur virus, a member of the species Sripur sripuvirus, was first isolated.

Sunrhavirus: from Sunguru, a village in the Arua District of Uganda from where Sunguru virus, a member of the species Sunguru sunrhavirus, was first isolated, and rhabdovirus.

Tibrovirus: from Tibrogargan virus, assigned to the species Tibrogargan tibrovirus.

Tupavirus: from the scientific name of the northern tree shrew (Tupaia belangeri) from which Tupaia rhabdovirus, a member of the species Tupaia tupavirus, was first isolated.

Varicosavirus: from varicose (Latin varix), meaning abnormal dilation or enlargement of a vein or artery, the symptom previously thought to be induced by lettuce big vein-associated virus (LBVaV). However, lettuce big-vein disease, although long thought to be induced by a virus previously designated lettuce big-vein virus, is now considered to be caused by members of Mirafiori lettuce big-vein ophiovirus (family Aspiviridae, genus Ophiovirus). Viruses of this species are soil-borne and often occur in lettuce together with isolates of LBVaV.

Vesiculovirus: from vesicular stomatitis and the associated lesions that can occur in the mouth and on hooves and udders of animals infected with some vesiculoviruses.

Zarhavirus: from Zahedan, Iran, where Zahedan virus was first isolated, and rhabdovirus.

Subfamily demarcation criteria

Viruses assigned to each subfamily form a monophyletic clade in well supported Maximum Likelihood (ML) or Maximum Clade Credibility (MCC) trees using full-length L sequences. Demarcation of subfamilies is based on significant differences in genome sequence and ecology as indicated by host range.

Genus demarcation criteria

Forty genera have been established to date. Viruses assigned to a genus form a monophyletic clade in well-supported Maximum Likelihood (ML) or Maximum Clade Credibility (MCC) trees using full-length L sequences.  The use of L for taxonomic purposes is justified by the presence of broadly conserved domains and the rarity of genetic recombination. Demarcation of genera is based upon considerations of significant differences in genome sequence and architecture, antigenicity and ecological properties (such as host range, pathobiology and transmission patterns). 

Relationships within the family

Phylogenetic relationships across the family have been established from ML or MCC trees generated from conserved regions of phylogenetically informative sequence in L (Figure 4.Rhabdoviridae). These can be identified by aligning full-length L sequences and eliminating ambiguously aligned regions using the Gblocks algorithm (http://molevol.cmima.csic.es/castresana/Gblocks_server.html). Phylogenetic relationships between viruses assigned to more closely related genera and within genera can also be established using other structural protein genes, notably N and G.

 

Figure 4.Rhabdoviridae. Maximum clade credibility (MCC) trees inferred from MUSCLE alignments of full-length rhabdovirus L sequences. A.  Sequences of 238 rhabdoviruses assigned to 37 genera generated in MEGA7 (Kumar et al., 2016). B. Sequences of the subset of 166 rhabdoviruses assigned to the subfamily Alpharhabdovirinae. Rhabdoviruses that have been assigned to species but for which full-length L sequences are not available are not included in the data set. Ambiguously aligned amino acid residues in each alignment were pruned using Gblocks (Castresana 2000). MCC trees were inferred in BEAST.v1.10.4 by using the Whelan and Goldman (WAG) model of amino acid substitutions, the gamma + invariant sites model of site heterogeneity and a strict molecular clock (coalescent: constant size) with a random starting tree to perform 10 million MCMC runs. The analysis was sampled at every 10000 states. Tree Annotator v1.10.4 was used to output the results of the MCC tree model and calculate posterior probabilities with a burn-in of 1 million states. FigTree was then used to plot the MCC phylogenetic tree. Branch lengths are drawn to scale with the scale bar showing the number of substitutions per site. The trees and corresponding sequence alignments are available to download from the Resources page.

Relationships with other taxa

Many general characteristics of rhabdovirus genome organisation, replication and transcription are shared with other members of the order Mononegavirales.

Related, unclassified viruses

Unclassified rhabdoviruses (additional unclassified rhabdoviruses that are probable members of existing genera are listed under individual genus descriptions).

Virus name

Accession number

Virus abbreviation

American dog tick rhabdovirus 1

MF360791

ADTRV-1

American dog tick rhabdovirus 2

MF962659, MF962661

ADTRV-2

anole lyssa-like virus 1

BR001666

ALLV-1

Apis rhabdovirus 1

KY354230

ApRV-1

Apis rhabdovirus 2

KY354233

ApRV-2

Atrato rhabdo-like virus 2

MN661109*

AtRLV-2

Atrato rhabdo-like virus 3

MN661034

AtRLV-3

Beaumont virus

KF310911*

BEAUV

Beihai dimarhabdovirus 1

MG600012

BhDRV-1

Beihai barnacle virus 7

KX884411

BhBV-7

Berant virus

KX580901*

BRTV

Blattodean rhabdo-related virus OKIAV14

MT153532

BlRRV-14

blue crab virus

not available

BCV

cereal chlorotic mottle virus

not available

CCMoV

Caledonia dog whelk rhabdo-like virus 2

MF190043*

CDWRLV

coleopteran rhabdo-related virus OKIAV10

MT153511

CoRRV-10

coleopteran rhabdo-related virus OKIAV20

MT153398

CoRRV-20

coleopteran rhabdo-related virus OKIAV28

MT153411

CoRRV-28

coleopteran rhabdo-related virus OKIAV29

MT153538*

CoRRV-29

Cururu virus

KY435948*

CURUV

DakArk 7292 virus

not available

-

Diachasminorpha longicaudata rhabdovirus

KP735609

DLonRV

Dielmo rhabdovirus

TSA#*

DMORV

dipteran rhabdo-like virus OKIAV5

MT153511

DiRRV-5

dipteran rhabdo-related virus OKIAV19

TSA#

DiRRV-19

dipteran rhabdo-related virus OKIAV27

TSA#*

DiRRV-27

dipteran rhabdo-related virus OKIAV36

TSA#*

DiRRV-36

drain fly rhabdovirus MG2015

not available

DFRV

Drosophila busckii sigmavirus

KR822813

DBusSV

Drosophila subobscura rhabdovirus

KR822817*

DSubRV

eastern sea garfish-associated rhabdo-like virus

MH716826*

 

eel virus B12

not available

EEV-B12

eel virus C26

not available

EEV-C26

entamoeba virus

not available

ENTAV

Eptesticus fuscus rhabdovirus

MT732687

EFusRV

Farmington virus

KC602379

FARV

fox fecal rhabdovirus

KF823814

FFRV

frog lyssa-like virus 1

MK473367

FLLV-1

Fujian dimarhabdovirus

MG600015

FjDRV

Grenada mosquito rhabdovirus 1

MH707450

GMRV-1

Guadeloupe culex rhabdovirus

MN013393

GLCRV

hemipteran rhabdo-related virus OKIAV26

MT153513*

HeRRV-26

hemipteran rhabdo-related virus OKIAV30

MT153400*

HeRRV-30

hemipteran rhabdo-related virus OKIAV47

MT153561*

HeRRV-47

Huangpi tick virus 3

KM817630

HpTV-3

Hubei dimarhabdovirus virus 2

KX884426

HbDRV-2

Hubei dimarhabdovirus virus 3

KX884423

HbDRV-3

Hubei dimarhabdovirus virus 4

KX884435*

HbDRV-4

Hubei myriapoda virus 7

KX884443

HbMV-7

Hubei rhabdo-like virus 1

KX884422

HbRLV-1

Hubei rhabdo-like virus 2

KX884447

HbRLV-2

Hubei rhabdo-like virus 5

KX884446*

HbRLV-5

Hubei rhabdo-like virus 6

KX884421*

HbRLV-6

Hubei rhabdo-like virus 8

KX884420*

HbRLV-8

hymenopteran rhabdo-related virus OKIAV1

MT153550

HyRRV-1

hymenopteran rhabdo-related virus OKIAV8

MT153471

HyRRV-8

hymenopteran rhabdo-related virus OKIAV25

MW288177*

HyRRV-25

hymenopteran rhabdo-related virus OKIAV40

MT153562*

HyRRV-40

hymenopteran rhabdo-related virus OKIAV45

MW288210*

HyRRV-45

Ixodes ricinus associated rhabdovirus

MT181988

IricRV

Jingshan fly virus 2

KM817631*

JsFV-2

lepidopteran rhabdo-related virus OKIAV3

MT153436*

LeRRV-3

lepidopteran rhabdo-related virus OKIAV11

MW288214*

LeRRV-11

Lye Green virus

KU754522*

LGV

mantodean rhabdo-related virus OKIAV15

TSA#

MaRRV-15

mecopteran rhabdo-like virus OKIAV42

TSA#*

MeRRV-42

midge-associated rhabdo-like virus 1M1C3

LR701656*

MaRRV-1

midge-associated rhabdo-like virus 2 M2C13

LR701642*

MaRLV-2

Myotis pequinius bat rhabdovirus

KJ641709*

MpBRV

mononegavirales sp

MH560586*

 

murine feces-associated rhabdovirus

MF175079

MuFARV

Nephotettix cincticeps negative-stranded RNA virus 1

MW084959

NcNSRV-1

neuropteran rhabdo-like virus OKIAV31

MW288224*

NeRRV-31

Norway mononegavirus 1

MF141072

NWMV-1

Norway mononegavirus 1-like virus

MH560586

Primus virus

MN567480

PRIMV

Pteromalus puparum negative-strand RNA virus 1

KX431032

PpNSRV

Rhipicephalus associated rhabdo-like virus

MH814974

RaRLV

Rhode Island virus

not available

RHIV

Rio Grande cichlid virus

not available

RGRCV

Rondonia rhabdovirus

MN560631*

RONRV

Sanxia water strider virus 5

KM817634

SxWSV-5

Schistocephalus solidus rhabdovirus

MN803433

SSolRV

Shuangao bedbug virus 2

KM817637

SgBV-2

Shuangao insect virus 6

KM817639*

SgIV-6

Sodak rhabdovirus 1

MT875151

SRV-1

sorghum stunt mosaic virus

not available

SSMV

soybean cyst nematode-associated northern cereal mosaic virus

HM849039

SNaRV

Taastrup virus

AY423355*

TAAV

Tacheng tick virus 3

KM817640*

TcTV-3

Tacheng tick virus 7

KM817642

TcTV-7

Taishun tick virus

KM817643

TsTV

Tetrastichus brontispae RNA virus 1

MH643740

TbRV-1

ulcerative disease rhabdovirus

not available

UDRV

Varroa jacobsoni rhabdovirus 1

MT482464

VjacRV-1

Wenling dimarhabdovirus 1

MG600014

WlDRV-1

Wenling dimarhabdovirus 8

MG600017

WlDRV-8

Wenling dimarhabdovirus 9

MG600018*

WlDRV-9

Wenling dimarhabdovirus 10

MG600019*

WlDRV-10

Withyham virus

KU754523*

WITHV

Wuhan insect virus 7

KM817653

WhIV-7

Wuhan louse fly virus 11

KM817658*

WhLFV-11

Wuhan mosquito virus 9

KM817659

WhMV-9

Wuhan pillworm virus 2

KX884442

WhPV-2

Xinzhou dimarhabdovirus virus 1

KX884460*

XzDRV-1

Virus names and virus abbreviations are not official ICTV designations.
* Coding region sequence incomplete
# TSA – transcriptome shotgun assembly. For viruses with OKIAV in their name see (Käfer et al., 2019). For Deilmo rhabdovirus see (Temmam et al., 2016)

Member taxa