Rhabdoviridae

Genus: Varicosavirus


Genus: Varicosavirus

Distinguishing features

Viruses assigned to the genus Varicosavirus form a monophyletic group based on well-supported Maximum Likelihood trees inferred from complete L sequences. Varicosaviruses infect plants. They have bi-segmented genomes and non-enveloped, flexuous, rod-shaped virions that, in electron micrographs, resemble nucleocapsids of other rhabdoviruses.

Virion

Morphology

Virions are fragile, non-enveloped rods mostly measuring 324–152 nm x approximately18 nm; each has a central canal approximately 3 nm in diameter and an obvious helix with a pitch of about 5 nm (Figure 1.Varicosavirus). The helix of particles, especially those in purified preparations, tends to loosen and particles are then seen as partially uncoiled filaments (Kuwata and Kubo 1981, Kuwata et al., 1983, Vetten et al., 1987).

Figure 1.Varicosavirus. Negative contrast electron micrograph of virions of an isolate of lettuce big vein-associated virus. The bar represents 100 nm. (Courtesy J.A. Walsh and C.M. Clay.)

Physicochemical and physical properties

Virus particles have a density in Cs2SO4 of about 1.27 g cm-3 (Kuwata et al., 1983).

Nucleic acid

The lettuce big vein-associated virus (LBVaV) genome consists of negative-sense, single-stranded RNA which is encapsidated by N (previously termed coat protein, CP). The genome (12.9 kb) occurs as two segments of 6.8 kb (RNA1) and 6.1 kb (RNA2) (Sasaya et al., 2002, Sasaya et al., 2004). The 3′ and 5′ terminal sequences of the two genome segments are similar but do not exhibit inverse complementarities. Compared with OFV (see genus Dichorhavirus), purified LBVaV virions contain a relatively large amount of anti-genomic RNAs (Sasaya et al., 2001). Conserved motifs in the transcription initiation and termination signals resemble those of viruses in the genus Cytorhabdovirus.

Proteins

RNA 2 encodes the 397-aa N with a predicted mass of 44.5 kDa (Mr approximately 48 kDa), as well as protein 2 (36 kDa), protein 3 (32 kDa), protein 4 (19 kDa) and protein 5 (41 kDa) which are of unknown functions but thought to be equivalent to P, P3, M and G of cytorhabdoviruses (Kormelink et al., 2011, Sasaya et al., 2004). RNA 1 encodes protein 6 (5 kDa), also of unknown function, and the 232 kDa L (Sasaya et al., 2002). N and L share a moderate amount of amino acid sequence identity with their counterparts in other rhabdoviruses.

Genome organisation and replication

RNA 1 contains two genes in the order 3′ 6-L 5′; RNA-2 contains five genes in the order 3′ N-2-3-4-5 5′ (Figure 2.Varicosavirus). Although the genome organisation of LBVaV is similar to that of other rhabdoviruses, LBVaV does not have a 3′ non-coding leader sequence. Capped and polyadenylated monocistronic mRNAs are transcribed from individual genes.

Figure 2.Varicosavirus. Schematic representation of the lettuce big vein-associated virus genome (RNA1 and RNA2). N and L represent ORFs encoding the structural proteins. ORFs numbered 2 to 6 encode putative proteins of unknown function. 

Antigenicity

N does not induce a strong antibody response in laboratory animals. LBVaV is closely related serologically to tobacco stunt virus (TStV), currently unclassified (Kuwata and Kubo 1986).

Biology

Varicosaviruses occur naturally in members of two families of plant species (Compositae and Solanaceae). LBVaV and TStV are transmitted in soil and in hydroponic systems by zoospores of the chytrid fungus Olpidium virulentus (a noncrucifer strain of Olpidium brassicae) (Hiruki 1967, Campbell 1962, Sasaya and Koganezawa 2006, Maccarone et al., 2010). LBVaV is frequently associated with lettuce big vein disease that is caused by Mirafiori lettuce big-vein virus (MLBVV; genus Ophiovirus, family Ophioviridae) (Roggero et al., 2000, Lot et al., 2002). Due to the longevity of infectious LBVaV from stored O. virulentus resting spores, it is assumed that the virus is carried internally by the fungus. The viruses are also transmitted experimentally, sometimes with difficulty, by mechanical inoculation (Huijberts et al., 1990). Neither of the viruses is reported to be seed-transmitted. LBVaV has been reported from Europe, United States, Japan, Saudia Arabia and Australia (Umar et al., 2017, Navarro et al., 2005, Maccarone et al., 2010, Alemzadeh and Izadpanah 2012).

Species demarcation criteria

LBVaV is assigned to the only species currently approved in the genus.

Member Species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus Abbreviation(s)
Lettuce big-vein associated varicosaviruslettuce big vein-associated virusRNA1: AB075039; RNA2: AB114138RNA1: NC_011558; RNA2: NC_011568Complete coding genomeLBVaV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.
Download GenBank/EMBL query for sequences listed in the table here.

Derivation of names

The genus name is derived from the symptom previously thought to be induced by LBVaV (Vari: from Latin varix, meaning abnormal dilation or enlargement of a vein or artery). However, lettuce big-vein disease, although long thought to be induced by a virus previously designated lettuce big-vein virus, is now considered to be caused by isolates of MLBVV (genus Ophiovirus). Viruses of this species are soil-borne and often occur in lettuce together with isolates of LBVaV.

Related, Unclassified Viruses 

Virus name

Accession number

Virus abbreviation

Alopecurus myosuroides varicosavirus 1

LN713933, LN713934

AMVV1

tobacco stunt virus

AB190521*

TStV

* Coding region sequence incomplete
Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.
Download GenBank/EMBL query for sequences listed in the table here.
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