Genus: Tibrovirus


Genus: Tibrovirus

Distinguishing features

Viruses assigned to the genus Tibrovirus form a distinct monophyletic group based on well-supported Maximum Likelihood trees inferred from complete L sequences. Several viruses assigned to the genus have been isolated from either biting midges (Culicoides spp.) or cattle; several other tibroviruses have been detected in humans.  The tibrovirus clade is part of a larger phylogenetic group of arthropod-borne rhabdoviruses with large and complex genomes that also includes ephemeroviruses, hapaviruses and curioviruses. Tibroviruses share the common features of: i) two additional genes (U1 and U2) located between the M gene and G gene; and ii) an additional gene (U3), between the G gene and L gene, encoding a class 1a viroporin-like protein.

Virion

Morphology

Bullet-shaped virions of varying morphology have been reported for tibroviruses. Long narrow particles (375 nm x 50 nm) were observed by negative-stain of freshly prepared cell culture supernates of Tibrogargan virus (TIBV) but similar samples stored for 1 week at 4oC revealed shorter tapered bullet-shaped particles (125 nm x 75 nm) (Cybinski et al., 1980). Long bullet-shaped particles (250 nm x 70 nm) have also been observed in ultrathin sections of mosquito cells infected with Bivens Arm virus (BAV) (species Tibrogargan tibrovirus) (Gibbs et al., 1989). 

Nucleic acid

Tibrovirus genomes consist of a single molecule of negative-sense, single-stranded RNA and range from approximately 12.5–13.3 kb (Gubala et al., 2011, Grard et al., 2012, Stremlau et al., 2015, Walker et al., 2015, Wiley et al., 2017).

Proteins

The N, P, M, G and L share sequence homology and/or structural characteristics with the cognate proteins of other rhabdoviruses (Walker et al., 2015, Gubala et al., 2011). The U1 genes encode proteins of unknown function that range from 170 to 216 amino acids (19.1–23.8 kDa); the U2 genes encode proteins of unknown function that range from 149 to 176 amino acids (17.1–20.1 kDa); the small viroporin-like proteins encoded in the U3 genes range from 92 to 125 amino acids (10.5–13.9 kDa) and feature an N-terminal domain containing large hydrophobic residues, a predicted hydrophobic transmembrane domain and a C-terminal domain that is rich in basic residues.

Genome organisation and replication

Tibrovirus genomes include five genes (N, P, M, G and L) encoding the structural proteins and multiple additional long ORFs including: i) two additional genes between the M and G genes encoding proteins of unknown function (U1 and U2); and ii) an additional gene between the G and L genes encoding a class 1a viroporin-like protein (U1) (Figure 1.Tibrovirus). All ORFs occur in discrete transcriptional units including consensus transcription initiation and transcription termination/polyadenylation sequences (Walker et al., 2015, Gubala et al., 2011).

Figure 1.Tibrovirus. Schematic representation of tibrovirus genome organisations. N, P, M, G and L represent ORFs encoding the structural proteins. ORF U1 (turquoise), ORF U2 (navy blue) and ORF U3 (yellow) are highlighted. ORF U1 and ORF U2 encode small proteins of unknown function and ORF U3 encodes a small viroporin-like transmembrane protein. 

Antigenicity

TIBV, Coastal Plains virus (CPV), BAV and Sweetwater Branch virus (SWBV) partially cross-react in indirect immunofluorescence, complement-fixation and/or neutralisation tests (Cybinski et al., 1980, Gibbs et al., 1989, Cybinski and Gard 1986).

Biology

Several tibroviruses (TIBV, CPV, BAV, SWBV, Beatrice Hill virus) infect cattle and/or water buffalo and have been isolated from biting midges (Culicoides spp.). Tibrovirus infection in cattle has been reported in Australia, Papua New Guinea, the USA and the Caribbean (Cybinski et al., 1980, Gibbs et al., 1989, Cybinski and Gard 1986). There has been no report of disease in ruminants associated with tibrovirus infection. Several other tibroviruses (Bas-Congo virus (BASV), Ekpoma virus 1 (EKV-1) and Ekpoma virus 2 (EKV-2) have been identified in human sera by next-generation sequencing but infectious virus isolates have not been recovered. BASV was detected in an acute serum sample from the lone survivor of an outbreak of haemorrhagic fever in the Democratic Republic of Congo (Grard et al., 2012). EKV-1 and EKV-2 were detected in blood taken from apparently healthy individuals in Nigeria (Stremlau et al., 2015). The role of tibroviruses in human disease is not currently clear.

Species demarcation criteria

Viruses assigned to different species within the genus Tibrovirus have several of the following characteristics: A) minimum amino acid sequence divergence of 20% in L; B) minimum amino acid sequence divergence of 20% in N; C) can be distinguished in serological tests; and D) occupy different ecological niches as evidenced by differences in hosts and/or arthropod vectors. All members clearly meet criteria A and B.  The viruses each meet the other criteria to varying extents based on available data. 

Member species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus abbreviationIsolate abbreviation
Bas-Congo tibrovirusBas-Congo virusJX297815Partial genomeBASV
Beatrice Hill tibrovirusBeatrice Hill virusCSIRO 25KY073493Complete genomeBHV
Coastal Plains tibrovirusCoastal Plains virus DPP53GQ294473NC_025397Complete genomeCPV
Ekpoma 1 tibrovirusEkpoma virus 1KP324827Partial genomeEKV1
Ekpoma 2 tibrovirusEkpoma virus 2KP324828Complete coding genomeEKV2
Sweetwater Branch tibrovirusSweetwater Branch virusUF-11KM204997NC_034546Complete coding genomeSWBV
Tibrogargan tibrovirusTibrogargan virusCS132GQ294472NC_020804Complete genomeTIBV
Tibrogargan tibrovirusBivens Arm virusUF-10KP688373BAV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.

Derivation of names

Tibrovirus: from the name of the virus assigned to the type species (Tibrogargan virus; TIBV; species Tibrogargan tibrovirus).