Genus: Lyssavirus


Genus: Lyssavirus

Distinguishing features

Viruses assigned to the genus Lyssavirus form a distinct monophyletic group based on well-supported maximum-likelihood trees inferred from complete L sequences. Lyssaviruses cause acute progressive encephalitis (rabies) in mammals, being transmitted between susceptible individuals directly by bites, scratches or contamination of mucous membranes with infected saliva, without participation of arthropod vectors. Bats (order Chiroptera) are the principal reservoir hosts for most lyssaviruses, whereas carnivores (order Carnivora), as well as bats, maintain circulation of rabies virus (RABV). Viruses assigned to the genus are distributed worldwide, except Antarctica and several isolated islands, although viruses of different species have different circulation ranges. Lyssavirus genomes contain only five structural protein genes (3′-N-P-M-G-L-5′) but feature a very long 3′-untranslated region following the G mRNA.

Virion

Morphology

The virions are bullet-shaped (60–110 nm x 130–250 nm), and composed of two structural units: an internal helical nucleocapsid, approximately 50 nm in diameter, and a lipid envelope which is derived from the host cytoplasmic membrane during budding (Hummeler et al., 1967). Knobbed spikes (8 nm in length), consisting of three glycosylated ectodomains of G, protrude through the virion membrane (Gaudin et al., 1992).

Physicochemical and physical properties

RABV particles consists of RNA (2–3%), protein (67–74%), lipid (20–26%) and carbohydrate (3%).

Nucleic acid

The single molecule of negative-sense single-stranded RNA is approximately 11.9–12.3 kb in length. The RNA is tightly associated with N within the RNP.

Proteins

The five major polypeptides include: N (Mr = 58–62 kDa), P (Mr = 35–40 kDa), M (Mr = 22–25 kDa), G (Mr = 65–80 kDa), and L (Mr = 190 kDa). RABV G is glycosylated and palmitoylated at sites that have been mapped (Gaudin et al., 1991, Wunner et al., 1985). N and P are both phosphoproteins and, in the case of RABV, phosphorylation involves different host protein kinases: cellular casein kinase II is implicated for N; and P is phosphorylated by certain isomers of protein kinase C as well as by an additional kinase, RVPK, yet to be clearly defined (Gupta et al., 2000). The N:P ratio in the RNP of RABV is 2:1 per virion, which indicates that two molecules of N interact with 1 molecule of P. M is not phosphorylated; it collaborates with both RNP and G in virion assembly to form bullet-shaped virions (Mebatsion et al., 1999). In infected cells, RABV N, P and M also have roles in modulating the host response to infection. N has been shown to inhibit retinoic acid-inducible gene I (RIG-I)-mediated activation of type 1 interferon (IFN) (Masatani et al., 2013, Masatani et al., 2010). Several isoforms of the RABV P (P1–P5) are expressed from five in-frame initiation codons (Chenik et al., 1995) and function to inhibit both interferon (IFN) induction and IFN-dependent signalling (Brzozka et al., 2005, Brzozka et al., 2006) by suppressing interferon regulatory factor 3 (IRF-3) and binding to signal transducers and activator of transcription 1 (STAT1) and STAT2 (Brzozka et al., 2006, Rieder et al., 2011, Vidy et al., 2005, Vidy et al., 2007). M modulates apoptosis (Kassis et al., 2004) and interacts with the necrosis factor (NF)-κB pathway to inhibit induction of IFN-β (Luco et al., 2012, Ben Khalifa et al., 2016). The lyssavirus virion envelope contains other host-derived minor protein components. A lipoprotein bilayer consists of a mixture of host-derived lipids, including phospholipids, neutral lipids and glycolipids.

Genome organisation and replication

The RABV genome consists of a 58 nt leader sequence, followed by five structural protein genes in the order 3′-N-P-M-G-L-5′, separated by non-transcribed intergenic regions and followed by a 57–70 nt trailer (Kuzmin et al., 2008) (Figure 1.Lyssavirus). Transcription initiation signal of each mRNA is conserved (3′-UUGUXR-5′), as well as the transcription termination-polyadenylation signal (3′-WCUUUUUUU-5′). Untranslated regions of mRNAs are relatively short, except the 3′-UTR of the G mRNA, which is approximately 440–700 nt in length. In West Caucasian bat virus (WCBV), there is an alternative ORF of 228 nt (76 amino acids) in the M gene and the long 3′-region of the G mRNA contains an ORF of 180 nt (60 amino acids) but it is not known if the corresponding proteins are expressed in infected cells (Walker et al., 2015, Kuzmin et al., 2008). Intergenic regions are variable (2–100 nt), but their lengths increase in the 3′-to-5′ direction, which has the potential effect of causing a progressive slowing and decreasing efficiency of transcription. RABV transcription and replication occur in the cytoplasm of infected cells and follow processes that are very similar to those described above for vesiculoviruses and other rhabdoviruses (Albertini et al., 2011).

Figure 1.Lyssavirus. Schematic representation of lyssavirus genome organisations. N, P, M, G and L represent ORFs encoding the structural proteins. The genomes feature long untranslated regions in the G genes (designated y in rabies virus); in West Caucasian bat virus this ~700 nt region contains a 180 nt ORF (turquoise) encoding a possible 7.1 kDa protein. West Caucasian bat virus also features an alternative ORF of 228 nt (orange), that commences near the start of the M gene, encoding a possible 8.4 kDa protein. It is not known if these proteins are expressed in infected cells.

Antigenicity

Based on antigenic properties and phylogenetic relationships, viruses in the genus have been subdivided into two phylogroups (Figure 1.Lyssavirus). Phylogroup I includes RABV, Australian bat lyssavirus (ABLV), Duvenhage virus (DUVV), European bat lyssavirus 1 (EBLV-1), European bat lyssavirus 2 (EBLV-2), Aravan virus (ARAV), Khujand virus (KHUV), Bokeloh bat lyssavirus (BBLV), Irkut virus (IRKV) and Gannoruwa bat lyssavirus (GBLV); phylogroup II includes Lagos bat virus (LBV), Mokola virus (MOKV) and Shimoni bat virus (SHIBV) (Badrane et al., 2001, Fooks 2004Gunawardena et al., 2016). The most divergent viruses in the genus, WCBV, Ikoma virus (IKOV) and Lleida bat lyssavirus (LLBV), are not members of either of these phylogroups (Marston et al., 2017, Marston et al., 2012, Horton et al., 2014, Weyer et al., 2008).

Antigenic cross-reactivity correlates with the relatively short genetic distances between lyssaviruses. Antigens of the RNP, which are most abundant in infected cells, cross-react between all members of the genus described to date (Rupprecht et al., 1991). This feature facilitates the use of standardised diagnostic reagents for detection of all lyssaviruses (e.g., by direct fluorescent antibody or immunohistochemical assays). In contrast, glycoprotein antigens are relatively conserved within phylogroups (ectodomain conservation >75%) but not between phylogroups (ectodomain conservation <65%). As a result, commercially available rabies vaccines and anti-rabies immunoglobulins, that mainly induce or provide neutralising antibodies targeting the glycoprotein, protect against phylogroup I lyssaviruses but not against other lyssaviruses (Badrane et al., 2001, Fooks 2004, Fekadu et al., 1988, Bahloul et al., 1998, Hanlon et al., 2005).

Biology

Lyssaviruses are essential neurotropic pathogens. Delivered into a wound via a bite or wound contamination, the virus can replicate at the inoculation site, as was shown for skeletal muscle cells (Murphy and Bauer 1974). The virus then reaches the motor or sensory neurons and propagates up to the central nervous system (CNS) by following neuronal connections and using retrograde axonal transport (Jackson 2013). Neuronal pathways shield the virus from host immune surveillance, resulting in absence of early antibody response (Johnson et al., 2010). Being delivered to the CNS, the virus disseminates rapidly. Nearly all regions of the CNS may be affected and RABV has been used to map neuronal connections (Ugolini 2011). The duration of the asymptomatic incubation period can be variable (two months on average), while the symptomatic clinical period is rapid and severe (about one week) (Udow et al., 2013). Neuropathological changes observed in the infected brain are relatively mild histologically and include gliosis, slight neuronophagia, perivascular infiltration with inflammatory cells, with rare involvement of meninges (Jackson 2013). Occasionally, more severe brain damage occurs, such as spongiform lesions, extensive neuronal degeneration and widespread inflammation (Jackson 2013). Functional alteration of the CNS is much more significant than is morphological representation. Reverse dissemination of virus from the CNS during the clinical period of rabies occurs along peripheral nerves. Viral RNA may be detected in a variety of organs and tissues at the end of the clinical period. However, only low titres of infectious virus occasionally can be isolated from extraneural tissues (Jackson et al., 1999). The exception is the salivary glands, in which virus undergoes additional replication cycles and is released into the saliva to complete the transmission (Jackson 2013). Acute generalised CNS dysfunction leads to a lethal outcome of the disease. Very rare cases of survival after manifestation of clinical signs of rabies have been observed in humans and some animals (Jackson et al., 2003). However, these sporadic events cannot be taken as support for a theory of lyssavirus persistence.

In nature, lyssaviruses are associated with specific mammalian reservoir hosts. Spill-over infections into vertebrates of other species lead to a dead end of the transmission chain in the vast majority of cases. The exception is RABV, which is distributed most broadly, and host shifts of certain variants, with the establishment of sustained circulation in a new host of a different species, have been well documented (Rupprecht et al., 2011, Badrane and Tordo 2001, Kuzmin et al., 2012, Troupin et al., 2016). Laboratory rodents (mice, hamsters), foxes and bats are highly susceptible to intra-cranial inoculation with lyssaviruses, whereas their susceptibility to peripheral inoculation varies, depending on viral species and lineage, inoculation dose and route (Fekadu et al., 1988, Vos et al., 2004, Eggerbauer et al., 2017). Cell cultures derived from the mammalian nervous tissue (such as mouse neuroblastoma, MNA cells) are more susceptible to lyssaviruses than are other mammalian cell cultures (BHK, Vero, etc.). Successful propagation in insect cell lines has been described only for MOKV (Buckley 1975).

Species demarcation criteria

Genetic distances between members of different lyssavirus species are significantly shorter than the distances between viruses of different species in other rhabdovirus genera, which has been attributed to evolutionary constraints, possibly imposed by their unique pathobiology or their vector/reservoir preferences.

Viruses assigned to different species within the genus Lyssavirus have several of the following characteristics:

  1. Genetic distances, with the threshold of 80–82% nucleotide identity for the complete N gene, that provides better quantitative resolution compared to other genes, or 80–81% nucleotide identity for concatenated coding regions of N+P+M+G+L Globally, all isolates belonging to the same species have higher identity values than the threshold, except the viruses currently assigned to the species Lagos bat lyssavirus. For that reason, some authors suggested that LBV isolates be subdivided into several genotypes. However, as these LBV representatives are segregated into a monophyletic cluster in most phylogenetic reconstructions, and in the absence of other sufficient demarcation characters there is currently no possibility to assign LBV isolates into different viral species.
  2. Topology and consistency of phylogenetic trees, obtained with various evolutionary models.
  3. Antigenic patterns in reactions with anti-nucleocapsid monoclonal antibodies (preceded by serologic cross-reactivity and definition of lyssavirus serotypes, using polyclonal antisera).
  4. Whenever available, additional characters, such as ecological properties, host and geographic range, pathological features are used.

Member Species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus Abbreviation(s)Isolate Abbreviation
Aravan lyssavirusAravan virus EF614259NC_020808Complete genomeARAV
Australian bat lyssavirusAustralian bat lyssavirus AF081020NC_003243Complete coding genomeABLV
Bokeloh bat lyssavirusBokeloh bat lyssavirus21961JF311903NC_025251Complete genomeBBLV
Duvenhage lyssavirusDuvenhage virus 86132SAEU293119NC_020810Complete genomeDUVV
European bat 1 lyssavirusEuropean bat lyssavirus 1RV9EF157976NC_009527Complete genomeEBLV-1
European bat 2 lyssavirusEuropean bat lyssavirus 2RV1333EF157977NC_009528Complete genomeEBLV-2
Gannoruwa bat lyssavirusGannoruwa bat lyssavirusRV3266KU244266NC_031988Complete genomeGBLV
Ikoma lyssavirusIkoma lyssavirusRV2508JX193798NC_018629Complete genomeIKOV
Irkut lyssavirusIrkut virus EF614260NC_020809Complete genomeIRKV
Khujand lyssavirusKhujand virus EF614261NC_025385Complete genomeKHUV
Lagos bat lyssavirusLagos bat virus 0406SENEU293108NC_020807Complete genomeLBV
Lleida bat lyssavirusLleida bat lyssavirusRV3208KY006983NC_031955Complete genomeLLEBV
Mokola lyssavirusMokola virus Y09762NC_006429Complete genomeMOKV
Rabies lyssavirusrabies virus M13215NC_001542Complete genomeRABV
Shimoni bat lyssavirusShimoni bat virus GU170201NC_025365Complete genomeSHIBV
West Caucasian bat lyssavirusWest Caucasian bat virus EF614258NC_025377Complete genomeWCBV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.
Download GenBank/EMBL query for sequences listed in the table here.

Derivation of names

Lyssavirusfrom Lyssa, the Greek goddess of madness, rage, and frenzy.

Related, Unclassified Viruses 

Virus name

Accession number

Virus abbreviation

Ozernoe lyssavirus

FJ905105

OZEV

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.
Download GenBank/EMBL query for sequences listed in the table here.