Genus: Lymphocystivirus

Genus: Lymphocystivirus

Distinguishing features

In contrast to the systemic infections seen with ranaviruses and megalocytiviruses, lymphocystivirus infections result in wart-like growths mainly on the skin of infected fish. These growths are composed of numerous, greatly enlarged, cells. Infected cells are commonly 100 μm in diameter and sometimes reach 1 mm. Lymphocystivirus-infected cells display a thick hyaline capsule, a central enlarged nucleus, and prominent basophilic DNA cytoplasmic inclusions.



Particle size varies from 198 to 227 nm for lymphocystis disease virus 1 (LCDV1) and 200 nm for lymphocystis disease virus 2 (LCDV2). The icosahedral capsid may show a fringe of fibril-like external protrusions about 2.5 nm in length. The presence of capsid fibers distinguishes lymphocystiviruses from members of the other two genera of vertebrate iridoviruses.

Physicochemical and physical properties

Virions are heat labile. Infectivity is sensitive to treatment with ether or glycerol.

Nucleic acid

Within the genus, genomes vary from 98 kbp (LCDV2) to 209 kbp (lymphocystis disease virus – Sparus auratus, LCDV-Sa), the last is the largest known genome among vertebrate iridoviruses. A contour length measurement of 146 kbp for LCDV1 suggests a terminal redundancy of approximately 50%. Like other vertebrate iridovirids, the lymphocystivirus genome is highly methylated (22%) with 5-methylcytosine at 74% of CpG. The complete DNA sequence is known for LCDV1, lymphocystis disease virus - China (LCDV-C), and LCDV-Sa, and partial sequences are known for several others. Surprisingly, the G+C content, but not the methylation status, of the three fully-sequenced lymphocystiviruses is more like that of invertebrate iridoviruses rather than other vertebrate iridoviruses (Table 2.Iridoviridae). Furthermore, the published genome of LCDV1 is much smaller than that of LCDV-C and LCDV-Sa. Whether this reflects critical biological differences between LCDV1 and LCDV-C/LCDV-Sa, or whether it reflects a sequencing error, perhaps due to the presence of repeat regions, and remains to be determined.


Little is known about the protein composition of lymphocystiviruses. The reported genomes are sufficient to encode between 108–183 proteins. BLAST analysis of predicted ORFs suggests that many putative gene products may be unique to the genus.

Genome organization and replication

The LCDV1 genome (103 kbp) contains 108 largely non-overlapping putative ORFs, 38 of which show significant homology to proteins of known function. These 38 ORFs represent 43% of the coding capacity of the genome. The presence of a DNA methyltransferase and a methyl-sensitive restriction endonuclease with specificity for a CCGG target site may be indicative of a restriction-modification system capable of degrading host genomic DNA while protecting viral DNA by specific methylation. LCDV1 DNA contains numerous short direct, inverted and palindromic repetitive sequence elements. LCDV2, isolated from dabs, possesses a genome estimated, by analysis of restriction enzyme fragments, to be 98 kbp. It has not yet been sequenced.

The LCDV-C genome contains 178 non-overlapping ORFs, 103 of which are homologs to the corresponding ORFs of LCDV1 and 75 potential genes that were not found in LCDV1 or other iridovirids. Among these 75 genes, there are eight genes that contain conserved domains of cellular genes and 67 novel genes that do not show any significant homology with sequences in public databases. Although LCDV1 and LCDV-C possess 103 genes in common, their gene order is markedly different. Furthermore, a large number of tandem and overlapping repeated sequences are observed in the LCDV-C genome.

The genome of LCDV-Sa contains 183 putative ORFs. BLASTp analysis found that 145 ORFs displayed significant similarity to other genes in the database. Of these 145, 129 best match those in LCDV-C, 10 best match LCDV1, two match ORFs within the scale drop disease virus (unclassified isolate, Megalocytivirus) genome and the remaining four best match either fish (3 ORFs) or spider (1 ORF) genes.

A lack of cell lines suitable for in vitro replication has hindered analysis of LCDV biogenesis. Because few studies have been conducted, it has been assumed that LCDV replication is generally similar to frog virus 3. Electron microscopy indicates that as with other iridovirids, virus assembly occurs in and around virogenic stroma within viral assembly sites.


The major capsid protein (MCP) is antigenic and protective antibodies are produced following exposure to this protein. There are other immunogenic viral proteins including one of 60 kDa for which a polyclonal antiserum provides a diagnostic reagent (Cano et al., 2006). In addition, monoclonal antibodies have been raised against several lymphocystivirus proteins and used in indirect immunofluorescence (IFA), Western blot, and enzyme-linked immunosorbent (ELISA) assays (Cheng et al., 2006).


Lymphocystis disease was the first clinical illness linked with an iridovirus. However, although clinical manifestations of infection (i.e., wart-like or tumor-like growths on the skin of infected fish) were first noted near the end of the 19th century, the specific etiological agent was not identified until nearly 100 years later.

LCDV1 and LCDV-C infect flounder and plaice, LCDV2 infects dab, and LCDV-Sa infects gilthead seabream. Isolates have also been obtained from other infected fish species, but their taxonomic position is unclear . Infection targets fibroblasts and results in wart-like lesions comprising grossly hypertrophied cells occurring mostly in the skin and fins (Flugel 1985). The disease has been observed in over 100 teleost species although members of viral species other than Lymphocystis disease virus 1 may cause a similar clinical disease. The duration of infected cell growth and viral proliferation is highly variable (5 days to 9 months) and is likely to be temperature-dependent. Virions are released following degeneration of the lesions. Transmission is achieved by contact; external sites, including the gills, are the principal portals of entry. High host population densities and external trauma are believed to enhance transmission. Implantation and injection are also effective routes of transmission. The incidence of disease may be higher in the presence of certain fish ectoparasites. LCDV is not considered to be of major economic importance in fish since mortality is low. However, although infections are usually benign and self-limiting, there may be commercial concerns because the unsightly appearance of infected fish leads to market rejection. Moreover, mortalities may occur when infections involve the gills or when there is debilitation or secondary bacterial infection.

Species demarcation criteria 

Definitive criteria have not yet been established to delineate viral species. In the future, species may be distinguished from one another by DNA sequence analysis and PCR. PCR primers targeted to regions within the MCP and ORF167L can be used to identify isolates.  

Phylogenetic analysis based on MCP, DNA polymerase and myristylated membrane protein genes indicates that lymphocystovirus isolates display considerable sequence diversity. Overall the genome of LCDV-Sa shares 55% identity to LCDV-C and 39% with LCDV1; LCDV1 and LCDV-C shared 42% identity to each other. Dot plot analysis of genomic co-linearity showed evidence of marked genomic rearrangements when compared to LCDV-C and almost complete lack of co-linearity with LCDV1. Based on sequence differences among these isolates and the marked lack of genomic co-linearity, LCDV1, LCDV-C, and LCDV–Sa may represent three distinct species within the genus. Other lymphocystivirus isolates from yellow perch, rockfish, pearl gourami, glass fish and paradise fish have been reported and partial sequence data is available. In view of the above, the number of species within the genus is currently under review.

Member species

SpeciesVirus name(s)Exemplar isolateExemplar accession numberExemplar RefSeq numberAvailable sequenceOther isolatesOther isolate accession numbersVirus abbreviationIsolate abbreviation
Lymphocystis disease virus 1lymphocystis disease virus 1L63545NC_001824Complete genomeLCDV1
Lymphocystis disease virus 2lymphocystis disease virus-ChinaChinaAY380826NC_005902Complete genomeLCDV-C
Lymphocystis disease virus 3lymphocystis disease virus-Sparus aurataSA9KX643370NC_033423Complete genomeLCDV-Sa

Virus names, the choice of exemplar isolates, and virus abbreviations, are not official ICTV designations.

Derivation of names

Isolates are named after the associated disease and the chronological order in which the sample was isolated (e.g., LCDV1), the geographic location of the isolate (e.g., LCDV-C, China), or the host from which it was isolated (e.g., LCDV-Sa, Sparus auratus (gilthead seabream)).

Related, unclassified viruses

Virus name

Accession numbera

Virus abbreviation

Genome (bp)



lymphocystis disease virus – China







lymphocystis disease virus – Sparus auratus






lymphocystitis disease virus-paradise fish






lymphocystis disease virus – painted glassfish






lymphocystis disease virus – pearl gourami






lymphocystis disease virus - rockfish






lymphocystis disease virus 2






Virus names and abbreviations are not official ICTV designations.
ND: not determined.
a Full genome sequences have been determined for LCDV-C and LCDV-Sa. For the other isolates, the major capsid protein gene sequence (LCDV-PF, -PaGl, -PeGr, and -RF) or a TNFαR-like protein (LCDV2) gene sequence have been determined.