Figure 1 (Left) Scheme showing the accessibility to antibodies of various parts of the coat protein amino acid (aa) sequence in particles of beet necrotic yellow vein virus (BNYVV). Encircled numbers designate different epitopes. (Center) Negative contrast electron micrograph of stained purified particles of BNYVV. (Right) From left (a) negative contrast electron micrograph of a BNYVV particle and (b, c, d) computer-filtered micrographs of BNYVV particles (courtesy of A.C. Steven, from Virology113, 428 (1981); with permission). The bar represents 100 nm.
Figure 2 Genome organization and translation strategies of beet necrotic yellow vein virus (BNYVV) and beet soil-borne mosaic virus (BSBMV). The scheme indicates a self-cleavage of the replicase protein (red arrow and black triangle), a suppressible UAG stop codon (yellow star), m7Gppp (black circle) and (An) the 3′ poly (A)-tails. Mtr, methyltransferase; Hel, helicase; Pro, protease; Pol, RNA polymerase; RT, readthrough; RTD, readthrough domain; sub, subgenomic; CRP, cysteine rich protein. N and p4.6 have never been detected. Nothing is known about BSBMV putative p11 and p13.
Figure 3 Phylogenetic (distance) tree based on the codon-aligned nucleotide sequences of the capsid (CP) and polymerase proteins of rod-shaped plant viruses. Numbers on branches indicate percentage of bootstrap support out of 10,000 bootstrap replications (when >60%). The scales indicate maximum composite likelihood distances. Trees produced in MEGA4. Viruses (or possible members) of the genus Benyvirus are highlighted. Other viruses are Genus Pomovirus: potato mop top virus (PMTV), beet soil-borne virus (BSBV), broad bean necrosis virus (BBNV); Furovirus: soil-borne cereal mosaic virus (SBCMV), soil-borne wheat mosaic virus (SBWMV); Tobamovirus: tobacco mosaic virus (TMV); Tobravirus: tobacco rattle virus (TRV); Hordeivirus: barley stripe mosaic virus (BSMV); Pecluvirus: Indian peanut clump virus (IPCV), peanut clump virus (PCV).