Figure 1 (A) Surface-shaded view of human astrovirus 1 (HAstV-1) reconstructed from 367 images recorded by electron cryo-microscopy and calculated at 30 Å resolution. The map displays a diameter of 440 Å and T=3 icosahedral symmetry (courtesy of Dr M. Yeager). (B) Negative contrast electron micrograph of virions of human astrovirus from a stool specimen. The bar represents 100 nm.
Figure 2 Organization of human astrovirus genomic RNA (gRNA) (HAstV-8). ORF 2 is expressed from a sgRNA detected in the cytoplasm of infected cells.
Figure 3 Genome organization of selected strains of astroviruses belonging to different host species.
Figure 4 Phylogenetic relationships within the family Astroviridae. The predicted amino acid sequences of the entire capsid polyprotein were aligned using CLUSTALW2. The phylogenetic tree was generated using the neighbor-joining algorithm implemented in the MEGA4 program.