Figure 1 Electron micrographs of sucrose density gradient purified RNPs of rice hoja blanca virus (RHBV). (Left) Small circular RNPs from the slowest sedimenting RHBV RNP. (Right) Larger, circular RNPs from the fastest sedimenting RHBV RNP. The bar represents 100 nm.
(Courtesy of A.M. Espinoza.)
Figure 2 Genome organization characteristic of (top) maize stripe virus (MSpV) and (bottom) rice grassy stunt virus (RGSV). Boxes indicate the positions and designations of the ORF translation products. V signifies virion-sense RNA and VC signifies virion-complementary sense RNA.
Figure 3 Diagram of the expression of the ambisense RNA of tenuiviruses. The black circle signifies the Cap, the broken line signifies non-viral nucleotides, V signifies virion-sense RNA and VC signifies virion-complementary sense RNA.
Figure 4 Phylogenetic tree showing the relationships among tenuiviruses. Input data were nt sequences of the coding regions of RNA-3 and -4 (RNA-5 and -6 respectively for RGSV) as aligned by PileUp (GCG-Wisconsin 9.0). A total of 3020 characters with clear positional homology across the alignment were used for the phylogenetic analysis. The phylogenetic tree was generated using Maximum Likelihood criteria as implemented by PAUP 4.0(b10), allowing for variable nucleotide substitution rates, rate heterogeneity between characters and rate heterogeneity between lineages. The tree was significantly superior to alternative trees, as determined by likelihood analysis. Bootstrap probabilities were calculated separately for the main tree and for the resolution among the strains of RHBV, RSV and RGSV. The following sequences were used: RHBV-co (AF004658, L14952); RHBV-cr (L07940, AF004657); EHBV (L75930, L48441); UHBV (U82447, U82446); RSV-jp(t) (X53563, D10979); RSV-jp(m) (D01094, D01039); RSV-cn (Y11095,Y11096); MSpV-us (M57426, L13438); RGSV-ph(l) (AB000403, AB000404); RGSV-ph(sc) (AB023779, AB023780); RGSV-cn (AF290947, AF287949).