Figure 1 Negative contrast electron micrograph (uranyl acetate) of virions of an isolate of Mirafiori lettuce big-vein virus. The bar represents 100 nm.
(Courtesy of R. G. Milne.)
Figure 2 Genome organization of genus Ophiovirus. Mirafiori lettuce big-vein virus (MiLBVV) is shown here. Boxes represents ORFs. The length of the RNA segments and the predicted sizes of the ORF products are indicated. V, viral RNA; vc, viral complementary RNA. RNA-4 is not reported for all ophioviruses.
(Modified from van der Wilk et al., 2002.)
Figure 3 Unrooted phylogenetic tree based on the predicted amino acid sequences (45aa) encoded by the conserved 136 nt fragment of the RdRp gene, obtained from 20 ophiovirus isolates belonging to the six species. Alignment was obtained using ClustalW, and analyzed by the neighbor-joining method, with 10 000 bootstrap replications. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test are shown next to the branches (when >50%). The evolutionary distances were computed using the Poisson correction method.
(Courtesy of A. M. Vaira.)
Figure 4 Unrooted phylogenetic tree of members of family Ophioviridae and other negative stranded RNA viruses based on their conserved RdRp modules. The tree was generated by the neighbor-joining method and bootstrap values (indicated for each branch node when >50%) were estimated using 1000 replicates. Viruses included in the analysis and the accession numbers used [ ] are: BDV [L27077], BEFV [AF234533], BUNV [X14383], CDV [NC_001921], CPsV [AY224663], DHOV [M65866], DUGV [U15018], FLUAV [J02151], FLUBV [M20170], FLUCV [M28060], HRSV [NC_001781], HTNV [X55901], IHNV [L40883], ISAV [AJ002475], LACV [U12396], LBVV [AB075039], LCMV [J04331], MARV [M92834], MEV [K01711], MiLV (MiLBVV) [AF525933], MuV [D10575], NCMV [NC_002251], NDV [X05399], NIV [AF212302], PUUV [M63194], RABV [M13215], RSV [D31879], RVFV [X56464], RWMV [AF335429], RYSV [NC_003746], SEOV [X56492], SeV [M19661], SNV [L37901], SYNV [L32603], TCRV [J04340], THOV [AF004985], TOSV [X68414], TRTV [U65312], TSWV [D10066], UUKV [D10759], VSIV [J02428], VSNJV [M20166], and ZEBOV [AF499101].
(Courtesy of S. Gago-Zachert and M. L. Garcia.)