Figure 1 (Left) Diagrammatic representation of an orthobunyavirus virion in cross-section. The surface spikes comprise two glycoproteins termed Gn and Gc (previously referred to as G1 and G2). The three helical nucleocapsids are circular and comprise one each of the unique ssRNA segments (L, large; M, medium; S, small) encapsidated by N protein and associated with the L protein
(courtesy of R. Pettersson).
Figure 2 Coding strategies of genome segments of members of the family Bunyaviridae.
Figure 3 Transcription and replication scheme of genome segments of members of the family Bunyaviridae for a negative-strand segment (left) and for an ambisense segment (right). The genome RNA and the positive sense viral complementary RNA, known as anti-genome RNA, are only found as ribonucleoprotein complexes and are encapsidated by N protein. The mRNA species contain host-derived primer sequences at their 5′ ends (•) and are truncated at the 3′ end relative to the vRNA template; the mRNAs are not polyadenylated. For the ambisense TSWV M and S RNA-derived subgenomic transcripts, termination occurs in the intergenic region, likely due to folding of a predicted AU-rich hairpin structure in nascent transcripts.
Figure 4 Electron micrograph of negatively stained particles of California encephalitis virus strain La Crosse virus. The bar represents 100 nm.
(Courtesy of D. H. L. Bishop.)
Figure 5 Electron micrographs of negatively stained particles of isolates of Hantaan virus (left, courtesy of C.S. Schmaljohn) and Tula virus (right, courtesy of S. Butcher and J. Hepojöki). The bars represent 100 nm.
Figure 6 Electron micrograph of negatively stained particles of Crimean-Congo hemorrhagic fever virus (CCHFV). The bar represents 100 nm.
(Courtesy of C. S. Schmaljohn.)
Figure 7 (Left) Electron micrograph of negatively stained particles of Rift Valley fever virus. The bar represents 50 nm
(courtesy of A. Freiburg and R. Flick).
Figure 8 Electron micrograph of negatively stained particles of Tomato spotted wilt virus (TSWV). The bar represents 100 nm.
(Courtesy of Dr Jan van Lent.)
Figure 9 Phylogenetic tree of aligned core polymerase domains from the L proteins of members of the family Bunyaviridae and from analogous proteins of other segmented (Arenaviridae, Orthomyxoviridae) negative strand RNA viruses. The tree was reconstructed using the Bayesian Monte Carlo Markov Chain method in BEAST (http://beast.bio.ed.ac.uk/). A maximum clade credibility tree is shown with mean branch lengths (substitutions per site), and Bayesian posterior probabilities given at the nodes.
(Courtesy of T. Sironen.)