Figure 1. (Left) Electron microscopic images of lymphocytic choriomeningitis virus (LCMV). (A) Thin section showing several virions budding from the surface of an infected BHK-21 cell. (B) Cryo-electron microscopic images of purified unstained virions frozen in vitreous ice. Arrowheads indicate glycoprotein spikes which are composed of trans-membrane GP2 and globular heads of GP1. The bar indicates 100 nm. (Courtesy R. Milligan, J. Burns and M. Buchmeier). (C) Diagrammatic representation of virion structure with trimeric spikes (Eschli et al., 2006; Schlie et al., 2010). L protein is the RNA polymerase; IB is inclusion bodies that could be ribosomes or could be related to self-assembling Z bodies (Kentsis et al., 2002).
(Courtesy C. Clegg and A. Featherstone, firstname.lastname@example.org.)
Figure 2 Organization, transcription and replication of the arenavirus L and S RNAs. Regions encoding the L, Z, GPC and N proteins are shown as boxes with arrowheads indicating the notional direction of translation. The intergenic regions separating the ORFs are indicated by gray boxes. RNA transcription processes are indicated by solid arrows.
Figure 3 Phylogenetic relationships among the Arenaviridae. N and L gene codon-aligned nt sequences of an isolate of each species (Table 1) were analyzed in MEGA 4 (maximum composite likelihood distances and 10,000 bootstrap replicates). Bootstrap values are shown at the branches if >80%.