Figure 1 (Left) Electron micrographs of Penicillium chrysogenum virus (PcV), the type species of the genus Chrysovirus. Samples were negatively stained in 2% uranyl acetate (lower panel) or prepared unstained and vitrified (upper panel); the bar represents 50 nm. (Right) Three-dimensional cryo-electron microscopy reconstruction of PcV virions at 8 Å resolution. Surface-shaded virion capsid viewed along an icosahedral twofold axis. Boundaries for two capsid proteins are outlined in black; each subunit has two similar domains (red and green), suggesting ancestral gene duplication. Icosahedral symmetry axes are indicated.
Figure 2 Genome organization of Penicillium chrysogenum virus (PcV). The genome consists of four dsRNA segments; each is monocistronic. The RdRp ORF (nt positions 145 to 3498 on dsRNA-1), the CP ORF (nt positions 158 to 3106 on dsRNA-2), the p3 ORF (nt positions 162 to 2900 on dsRNA-3) and the p4 ORF (nt positions 163 to 2706 on dsRNA-4) are represented by rectangular boxes.
Figure 3 Neighbor-joining phylogenetic tree constructed based on the complete amino acid sequences of RdRps of members and probable members of the family Chrysoviridae and selected viruses from the family Totiviridae. The amino acid sequences were aligned using the program CLUSTAL W. See text for names and abbreviations of chrysoviruses. The following viruses in the family Totiviridae were included in the phylogenetic analysis (abbreviations in parenthesis): Gremmeniella abietina RNA virus L (GaRV-L), Helminthosporium victoriae virus 190S (HvV190S), Magnaporthe oryzea virus 2 (MoV2), Saccharomyces cerevisiae virus L-A (ScV-L-A), and ScV-L-BC, Sphaeropsis sapinea RNA virus 1 (SsRV1), Trichomonas vaginalis virus 1 (TVV1) and Ustilago maydis virus H1 (UmV-H1). The phylogenetic tree was generated using the MEGA4 phylogenetic package. Bootstrap values as a percent of 2000 replicates are indicated at each node.