Since only one genus is currently recognized, the family description corresponds to the genus description.
Type species Sulfolobus newzealandicus droplet-shaped virus
Sulfolobus newzealandicus droplet-shaped virus (SNDV) virions are somewhat pleiomorphic, with a droplet shape 75–90×110–185 nm with a beard of dense filaments at the pointed end.
Virions are unstable in CsCl and lyse.
The genome is covalently-closed circular dsDNA about 20 kbp. The genome cannot be cut by many restriction endonucelases, but can be cut by the dam-methylation dependent restriction endonuclease DpnI, indicating that it is extensively methylated by a dam-like methylase.
By SDS-PAGE analysis, there is one major virion protein, 17.5 kDa, and two minor virion proteins of 13.5 and 13 kDa.
Negative contrast electron microscopy of particles of Sulfolobus newzealandicus droplet-shaped virus (SNDV). The bar represents 100 nm.
(Courtesy of W. Zillig.)
SNDV exclusively infects Sulfolobus isolates from New Zealand, including the strain STH1/3. Virus production starts in the early stationary phase.
Sulfolobus newzealandicus droplet-shaped virus
Sulfolobus newzealandicus droplet-shaped virus-NZ
Species names are in italic script; names of isolates are in roman script. Sequence accession numbers [ ] and assigned abbreviations ( ) are also listed.
Gutta: from Latin gutta, “droplet”.
Arnold, H.P., Ziese, U. and Zillig, W. (2000). SNDV, a novel virus of the extremely thermophilic and acidophilic archaeon Sulfolobus. Virology, 272, 409-416.
Prangishvili, D., Stedman, K. and Zillig, W. (2001). Viruses of the extremely thermophilic archaeon Sulfolobus. Trends Microbiol., 9, 39-43.
Zillig, W., Arnold, H.P., Holz, I., Prangishvili, D., Schweier, A., Stedman, K., She, Q., Phan, H., Garrett, R. and Kristjansson, J.K. (1998). Genetic elements in the extremely thermophilic archaeon Sulfolobus. Extremophiles, 2, 131-140.
Zillig, W., Reiter, W.D., Palm, P., Gropp, F., Neumann, H. and Rettenberger, M. (1988). Viruses of archaebacteria. In: Calendar, R. (ed.), The Bacteriophages, Vol. 1. Plenum Press, New York, pp. 517-558.
In reproducing this chapter from the Eighth ICTV Report, the editors gratefully acknowledge the contribution of the author, Stedman, K.M.