Figure 1 Baculovirus occlusion bodies, virions and nucleocapsids. (Upper left) The structures of occlusion bodies from baculoviruses in the genera Alphabaculovirus (nucleopolyhedrovirus, NPV) and Betabaculovirus (granulovirus, GV) are illustrated. Virions embedded in nucleopolyhedrovirus occlusion bodies may contain multiple nucleocapsids (MNPV) or single nucleocapsids (SNPV). (Upper right) The two baculovirus virion phenotypes are illustrated as diagrams with shared and phenotype-specific components (from Blissard, 1996). (Bottom) Transmission electron micrographs of occlusion bodies (MNPV, SNPV and GV), virion phenotypes BV (budded virions), ODV (occlusion-derived virions) and nucleocapsids (NC). Nucleopolyhedrovirus occlusion bodies of the MNPV (Autographa californica MNPV, top left) and SNPV (Trichoplusia ni SNPV, top middle) types are compared to granulovirus occlusion bodies (Estigmine acrea GV, top right). Transmission electron micrographs of virions of the BV (Lymantria dispar MNPV, bottom left) and ODV (Autographa californica MNPV, bottom center) phenotypes are shown beside negatively stained nucleocapsids (Autographa californica MNPV, bottom right).
(Electron micrographs courtesy of J.R. Adams [LdMNPV BV virion] and R. Granados [all others].)
Figure 2 Transmission electron micrographs of occlusion bodies from the genera (A) Gammabaculovirus (Neodiprion abietis nucleopolyhedrovirus, courtesy of C.J. Lucarotti) and (B) Deltabaculovirus
(Culex nigripalpus nucleopolyhedrovirus, courtesy of J.J. Becnel).
Figure 3 The covalently closed circular genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is illustrated with locations and orientations of known and predicted ORFs (arrows). Restriction maps for EcoRI and HindIII are indicated by letters on outer and inner rings, respectively. Locations of homologous repeat (hr) sequences are indicated on the inside of the circle as small filled boxes. Map units are indicated on the inside of the map (1 map unit = 1.339 kbp)
(redrawn from Ayres et al. (1994). Virology, 202, 586–605). (See Rohrmann, 2011 for details.)
Figure 4 Phylogeny of the Baculoviridae. The maximum likelihood tree, based on the alignment of 30 genes, shows the relationships of the 39 species for which a completely annotated genome was available at time of analyses. Abbreviations are defined in lists of species and related viruses above.