Figure 1 (a) Diagram of extracellular ASFV virions showing nucleoid, matrix or inner core shell, capsid and lipid envelopes. (b) EM image of extracellular virions. Black arrow is outer envelope, white arrow is virus membrane. Bar=200 nm. The preparation method was standard chemical fixation for EM. (c) EM image of intracellular virions. IM=immature virion, M=mature virion. Black arrow is capsid protein, white arrow is virus membrane. Bar=200 nm. Preparation method was high pressure freezing followed by freeze substitution. (d) EM image of intracellular virions. Black arrow is capsid protein, white arrow is virus membrane. Bar=200 nm. Preparation method was thawed cryo-sections stained with uranyl acetate.
(Images kindly provided by Pippa Hawes, Institute for Animal Health, UK.)
Figure 2 Phylogenetic analysis of ASFV sequences for RNA polymerase, compared to corresponding sequences from dsDNA viruses and non-viral sequences. Sequences are shown in color as follows: asfarviruses, red; mimivirus, brown; poxviruses, purple; phycodnaviruses, green; ascoviruses, orange; iridoviruses, blue; non-viral Blast matches, yellow. Bootstrap values over 65% (>650) are shown. Abbreviations: APMV, Acanthamoeba polyphaga mimivirus; ATCV1, Acanthocystis turfacea chlorella virus 1; CIV, Chilo iridescent virus; DpAV4, Diadromus pulchellus ascovirus 4; G. anomala, Glugea anomala; H. andersenii, Hemiselmis andersinii; HvAV3, Heliothis virescens ascovirus 3; I. scapularis, Ixodes scapularis; ISKNV, infectious spleen and kidney necrosis virus; K. JI2008, Kabatana sp. strain JI2008; L. acerinae, Loma acerinae; LDV, lymphocystis disease virus; LSDV, lumpy skin disease virus; MsEPV, Melanoplus sanguinipesentomopoxvirus; OSGIV, orange-spotted grouper iridovirus; SfAV1, Spodoptera frugiperda ascovirus 1; SGIV, Singapore grouper iridovirus; TFV, tiger frog virus; TnAV2c, Trichoplusia ni ascovirus 2c; YMTV, Yaba monkey tumor virus, Variola, ASFVL African swine fever virus-like sequence.
(Figure redrawn from Loh, J., Zhao, G., Presti, R.M., Holtz, L.R., Finkbeiner, S.R., Droit, L., Villasana, Z., Todd, C., Pipas, J.M., Calgua, B., Girone, R., Wang, D. and Virgin, H.W. (2009). Detection of novel sequences related to African swine fever virus in human serum and sewage. J. Virol., 83, 13019–13025.)